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Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RAD54.
(Peptide available as ab90365.)
Our Abpromise guarantee covers the use of ab10705 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 70 kDa (predicted molecular weight: 84.3 kDa).|
|IP||Use a concentration of 5 µg/ml.|
Rabbit polyclonal to RAD54 (ab10705) at 1/500.
Lane 1 Marker
Lane 2 HeLa nuclear extract
Lane 3 HeLa cell lysate
Lane 4 A432 cell lysate
Lane 5 MCF7 cell lysate
Lane 6 HEK 293 cell lysate
Secondary antibody - Goat polyclonal to rabbit IgG (HRP) - ab6721.
RAD54 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to RAD54 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab10705.
Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).
Band: 70kDa; RAD54, non specific band: 75 kDa, We are unsure as to the identity of this extra band. 99kDa band:due to background seen in No ab control lane (2).
ab10705 has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"