Overview
- Product nameAnti-RAE1 antibody [EPR6923]See all RAE1 primary antibodies ...
- DescriptionRabbit monoclonal [EPR6923] to RAE1
- Tested applicationsIP, WB, IHC-P more details
- Species reactivityReacts with: Human
Does not react with
Mouse, Rat - Immunogen
Synthetic peptide corresponding to residues in Human RAE1.
- Positive controlMCF7, HeLa, HepG2, 293T and SH-SY5Y cell lysates; Human kidney tissue.
- General notesProduced under U.S. Patent No. 5,675,063.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Dissociation constant (Kd) Kd = 6.50 x 10-11 M
- Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
- PurityTissue culture supernatant
- Clonality Monoclonal
- Clone numberEPR6923
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab124783 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IP | IP: Use at an assay dependent concentration. |
| WB | WB: 1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa). |
| IHC-P | IHC-P: Use at an assay dependent dilution. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Heat up to 98 degrees C, below boiling, and then let cool for 10-20 min |
Target
- FunctionBinds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.
- Sequence similaritiesBelongs to the WD repeat rae1 family.
Contains 4 WD repeats. - Cellular localizationCytoplasm. Nucleus.
-
Database links
- Entrez Gene: 8480 Human
- Omim: 603343 Human
- SwissProt: Q3SYL7 Human
- SwissProt: P78406 Human
- Unigene: 371698 Human
Target information above from: UniProt accession
P78406
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- dJ481F12.3 antibodydJ800J21.1 antibodyFLJ30608 antibody
- Homolog of yeast Rae1 (Bharathi) mRNA associated protein of 41 kDa (Kraemer) antibodyHomolog of yeast Rae1 mRNA associated protein of 41 kDa antibodyMGC117333 antibodyMGC126076 antibodyMGC126077 antibodyMIG 14 antibodyMIG14 antibodyMigration inducing gene 14 antibodyMnrp 41 antibodyMnrp41 antibodymRNA associated protein mrnp 41 antibodymRNA binding protein 41 kD antibodymRNA export factor antibodymRNA export protein antibodymRNA-associated protein mrnp 41 antibodyMRNP 41 antibodyMRNP41 antibodyRAE 1 antibodyRAE1 (RNA export 1 S.pombe) homolog antibodyrae1 antibodyRAE1 homolog antibodyRae1 protein homolog antibodyRAE1 RNA export 1 homolog (S. pombe) antibodyRAE1 RNA export 1 homolog antibodyRAE1L_HUMAN antibodyRNA export 1 antibodyRNA export 1 homolog antibody
see all
Anti-RAE1 antibody [EPR6923] images
-
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAE1 antibody [EPR6923] (ab124783)ab124783 at 1/100 dilution staining RAE1 in paraffin-embedded Human kidney tissue by Immunohistochemistry.
-
All lanes : Anti-RAE1 antibody [EPR6923] (ab124783) at 1/1000 dilution
Lane 1 : MCF7 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : 293T cell lysate
Lane 5 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat anti-Rabbit HRP at 1/2000 dilution
Predicted band size : 41 kDa -
Equilibrium disassociation constant (KD)
Learn more about KD
Click here to learn more about KD
References for Anti-RAE1 antibody [EPR6923] (ab124783)
ab124783 has not yet been referenced specifically in any publications.

 Other-Anti-RAE1 antibody [EPR6923](ab124783)](http://a.abcam.com/ps/datasheet/images/124/ab124783/RAE1-Primary-antibodies-ab124783-3.jpg)