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Western blot - RAE1 antibody (ab36139)
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Product Name
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RAE1 antibody
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Product type
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Primary antibodies
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Description
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Goat polyclonal to RAE1
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Immunogen
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Synthetic peptide: NAAEELKPRNKK, corresponding to C terminal amino acids 357-368 of Human RAE1
BLAST 'NAAEELKPRNKK' with  or 
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Reacts with
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Hu
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Predicted to react
(species key)
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Ms, Rat, Cow, Xl, Zfsh
(Due to sequence homology)
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Tested applications
(see key)
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ELISA, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/64000.
WB: Use at a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 38-40 kDa observed in lysates of Human Hepatocarcinoma cell line HepG2 (predicted molecular weight: 41 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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RAE1 has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm.
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Pore
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
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Relevance
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Mutations in Rae1 and Gle2(Saccharomyces cerevisae homolog) genes have been shown to result in accumulation of poly(A)-containing mRNA in the nucleus, suggesting that the encoded proteins are involved in RNA export. It contains four WD40 motifs, and has been shown to localize to distinct foci in the nucleoplasm, to the nuclear rim, and to meshwork-like structures throughout the cytoplasm. This gene is thought to be involved in nucleocytoplasmic transport, and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton. Depletion of Rae1 from extracts or cells severely inhibits mitotic spindle assembly. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, TBS. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RAE1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RAE1 antibody images, references, products related to ab36139 and other tools.
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RAE1 antibody images:
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 Western blot - RAE1 antibody (ab36139)
RAE1 antibody (ab36139) at 0.03 µg/ml + HepG2 lysate(RIPA buffer, 30µg total protein per lane).
Predicted band size : 41 kDa
Observed band size : 41 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
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References for RAE1 antibody (ab36139)
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This product has been used in:
Cuende J et al. Retinoic acid downregulates Rae1 leading to APC(Cdh1) activation and neuroblastoma SH-SY5Y differentiation. Oncogene 27:3339-44 (2008). WB. PubMed: 18212744
If you publish research using ab36139 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to RAE1
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RAE1 antibody - more information
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Customer reviews (feedback) regarding RAE1 antibody |
Customer FAQs regarding RAE1 antibody |
Protocols for RAE1 antibody |
Price and availability of products related to RAE1 antibody |
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RAE1 antibody - related products:
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RAE1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RAE1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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