Overview

  • Product nameAnti-RAE1 antibody
    See all RAE1 primary antibodies
  • Description
    Goat polyclonal to RAE1
  • Tested applicationsSuitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    NAAEELKPRNKK

    , corresponding to C terminal amino acids 357-368 of Human RAE1

  • Positive control
    • HepG2, HeLa, Jurkat, MCF7, Human brain cortex

Properties

Applications

Our Abpromise guarantee covers the use of ab36139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38-40 kDa (predicted molecular weight: 41 kDa).
IHC-P Use a concentration of 3 - 6 µg/ml.

Target

Anti-RAE1 antibody images

  • All lanes : Anti-RAE1 antibody (ab36139) at 0.1 µg/ml

    Lane 1 : HepG2
    Lane 2 : HeLa
    Lane 3 : Jurkat
    Lane 4 : MCF7

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    35µg protein in RIPA buffer

    Primary incubation was 1 hour

    Detected by chemiluminescence

  • Anti-RAE1 antibody (ab36139) at 0.03 µg/ml + HepG2 lysate (RIPA buffer) at 30 µg

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

    Primary incubated for 1 hour. Detected using chemiluminescence.

  • ab50411 at 3.8 µg/ml staining RAE1 in Human brain cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

References for Anti-RAE1 antibody (ab36139)

This product has been referenced in:
  • Cuende J  et al. Retinoic acid downregulates Rae1 leading to APC(Cdh1) activation and neuroblastoma SH-SY5Y differentiation. Oncogene 27:3339-44 (2008). WB . Read more (PubMed: 18212744) »

See 1 Publication for this product

Product Wall

There are currently no Abreviews or Questions for ab36139.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"