Overview

  • Product name
  • Description
    Goat polyclonal to RAE1
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Xenopus laevis, Zebrafish
  • Immunogen

    Synthetic peptide:

    NAAEELKPRNKK

    , corresponding to C terminal amino acids 357-368 of Human RAE1

  • Positive control
    • HepG2, HeLa, Jurkat, MCF7, Human brain cortex

Properties

Applications

Our Abpromise guarantee covers the use of ab36139 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38-40 kDa (predicted molecular weight: 41 kDa).
IHC-P Use a concentration of 3 - 6 µg/ml.

Target

Images

  • All lanes : Anti-RAE1 antibody (ab36139) at 0.1 µg/ml

    Lane 1 : HepG2
    Lane 2 : HeLa
    Lane 3 : Jurkat
    Lane 4 : MCF7

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 41 kDa
    Observed band size : 40 kDa (why is the actual band size different from the predicted?)

    35µg protein in RIPA buffer

    Primary incubation was 1 hour

    Detected by chemiluminescence

  • Anti-RAE1 antibody (ab36139) at 0.03 µg/ml + HepG2 lysate (RIPA buffer) at 30 µg

    Predicted band size : 41 kDa
    Observed band size : 41 kDa

    Primary incubated for 1 hour. Detected using chemiluminescence.

  • ab50411 at 3.8 µg/ml staining RAE1 in Human brain cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.

References

This product has been referenced in:
  • Cuende J  et al. Retinoic acid downregulates Rae1 leading to APC(Cdh1) activation and neuroblastoma SH-SY5Y differentiation. Oncogene 27:3339-44 (2008). WB . Read more (PubMed: 18212744) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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