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Products:Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Overview
Product name
Anti-RAE1 antibody
See all RAE1 products (4) ...
Description
Goat polyclonal to RAE1
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse, Rat, Cow, Dog, Pig, Xenopus laevis, Zebrafish
Immunogen
Synthetic peptide: NAAEELKPRNKK, corresponding to C terminal amino acids 357-368 of Human RAE1
NAAEELKPRN KK
Positive control
Human brain cortex Human placenta lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris buffered saline, pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> RNA Processing >> Other
Signal Transduction >> Protein Trafficking >> Nuclear Import / Export
Tags & Cell Markers >> Subcellular Markers >> Nucleus >> Nuclear Pore
Cell Biology >> Cell Cycle >> Cell Division >> Spindle
Applications
Show applications key
Our Abpromise guarantee covers the use of ab36139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 0.03 - 0.1 µg/ml.Detects a band of approximately 38-40 kDa (predicted molecular weight: 41 kDa).
IHC-P: Use a concentration of 3 - 6 µg/ml.
Target
Function
Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.
Sequence similarities
Belongs to the WD repeat rae1 family.
Contains 4 WD repeats.
Cellular localization
Cytoplasm. Nucleus.
Target information above from: UniProt accessionP78406
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- dJ481F12.3 antibody
- dJ800J21.1 antibody
- FLJ30608 antibody
see all
Database links
- Entrez Gene: 513080 Cow
- Entrez Gene: 485942 Dog
- Entrez Gene: 8480 Human
- Entrez Gene: 66679 Mouse
- Entrez Gene: 100125839 Pig
- Entrez Gene: 362281 Rat
- Entrez Gene: 393973 Zebrafish
- Omim: 603343 Human
see all
Anti-RAE1 antibody images:
Western blot - RAE1 antibody (ab36139)
Anti-RAE1 antibody (ab36139) at 0.03 µg/ml + HepG2 lysate(RIPA buffer, 30µg total protein per lane).
Predicted band size : 41 kDa
Observed band size : 41 kDa
Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAE1 antibody (ab36139)
ab50411 at 3.8 µg/ml staining RAE1 in Human brain cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.
References for Anti-RAE1 antibody (ab36139)
This product has been referenced in:
- Cuende Jet al. Retinoic acid downregulates Rae1 leading to APC(Cdh1) activation and neuroblastoma SH-SY5Y differentiation. Oncogene 27:3339-44 (2008). WB.Read more (PubMed: 18212744) »
See 1 publication for this product
Publishing research using ab36139? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"