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Product name Anti-RAE1 antibody See all RAE1 primary antibodies
Goat polyclonal to RAE1
Tested applications Suitable for:
WB, IHC-P more details
Predicted to work with:
Mouse, Rat, Cow, Dog, Pig, Xenopus laevis, Zebrafish
NAAEELKPRNKK , corresponding to C terminal amino acids 357-368 of Human RAE1
HepG2, HeLa, Jurkat, MCF7, Human brain cortex
Storage instructions Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, 5mg/ml Tris buffered saline, pH 7.3
Concentration information loading...
Purity Immunogen affinity purified
Purification notes Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 - 0.3 µg/ml. Detects a band of approximately 38-40 kDa (predicted molecular weight: 41 kDa).
Use a concentration of 3 - 6 µg/ml.
Function Binds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.
Sequence similarities Belongs to the WD repeat rae1 family. Contains 4 WD repeats.
Cellular localization Cytoplasm. Nucleus.
Information by UniProt
Anti-RAE1 antibody images
Western blot - Anti-RAE1 antibody (ab36139)
All lanes : Anti-RAE1 antibody (ab36139) at 0.1 µg/ml Lane 1 : HepG2 Lane 2 : HeLa Lane 3 : Jurkat Lane 4 : MCF7 Lysates/proteins at 35 µg per lane. Predicted band size : 41 kDa Observed band size : 40 kDa ( why is the actual band size different from the predicted?)
35µg protein in RIPA buffer
Primary incubation was 1 hour
Detected by chemiluminescence
Western blot - RAE1 antibody (ab36139)
Anti-RAE1 antibody (ab36139) at 0.03 µg/ml + HepG2 lysate (RIPA buffer) at 30 µg Predicted band size : 41 kDa Observed band size : 41 kDa
Primary incubated for 1 hour. Detected using chemiluminescence.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RAE1 antibody (ab36139)
ab50411 at 3.8 µg/ml staining RAE1 in Human brain cortex by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Steamed antigen retrieval with citrate buffer pH 6 was performed. Antibody was revealed with AP-staining.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"