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Overview

  • Product nameRAE1 protein (Tagged-His Tag)See all RAE1 proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 76-368 of Human RAE1 with N terminal His tag; 293 amino acids, 36kDa (33.2kDa predicted from sequence). UniProt ID = P78406.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 97 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesQTIPKAQQMHTGPVLDVCWSDDGSKVFTASCDKTAKMWDL SSNQAIQIAQHDAPVKTIHW IKAPNYSCVMTGSWDKTL KFWDTRSSNPMMVLQLPERCYCADVIYPMAVVATAERGLI VY QLENQPSEFRRIESPLKHQHRCVAIFKDKQNKPTGF ALGSIEGRVAIHYINPPNPAKDNF TFKCHRSNGTNTSA PQDIYAVNGIAFHPVHGTLATVGSDGRFSFWDKDARTKLK TSEQLD QPISACCFNHNGNIFAYASSYDWSKGHEFYNP QKKNYIFLRNAAEELKPRNKK
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92112 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    MS
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        dJ481F12.3dJ800J21.1FLJ30608
        Homolog of yeast Rae1 (Bharathi) mRNA associated protein of 41 kDa (Kraemer)Homolog of yeast Rae1 mRNA associated protein of 41 kDaMGC117333MGC126076MGC126077MIG 14MIG14Migration inducing gene 14Mnrp 41Mnrp41mRNA associated protein mrnp 41mRNA binding protein 41 kDmRNA export factormRNA export proteinmRNA-associated protein mrnp 41MRNP 41MRNP41RAE 1rae1RAE1 (RNA export 1 S.pombe) homologRAE1 homologRae1 protein homologRAE1 RNA export 1 homologRAE1 RNA export 1 homolog (S. pombe)RAE1L_HUMANRNA export 1RNA export 1 homolog
      see all
  • FunctionBinds mRNA. May function in nucleocytoplasmic transport and in directly or indirectly attaching cytoplasmic mRNPs to the cytoskeleton.
  • Sequence similaritiesBelongs to the WD repeat rae1 family.
    Contains 4 WD repeats.
  • Cellular localizationCytoplasm. Nucleus.

    • Target information above from: UniProt accession P78406 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    RAE1 protein (Tagged-His Tag) images

    • SDS-PAGE - RAE1 protein (Tagged-His Tag) (ab92112)
      SDS-PAGE - RAE1 protein (Tagged-His Tag) (ab92112)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RAE1 protein (Tagged-His Tag) (ab92112)

    ab92112 has not yet been referenced specifically in any publications.

    Publishing research using ab92112 ? Please let us know so that we can cite the reference in this datasheet

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