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Overview

  • Product nameRAGE Human ELISA KitSee all RAGE kits ...
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 3 pg/ml
  • Range
    2.06 pg/ml - 1500 pg/ml
  • Recovery

    90 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 103.4 94% - 112%
    Serum 89.25 79% - 98%
    Plasma 78.58 67% - 93%

  • Species reactivity
    Reacts with: Human
  • Product overview

    ab100632 RAGE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human RAGE in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for human RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applicationsELISA more details

Properties

  • FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • Tissue specificityEndothelial cells.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession Q15109 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Advanced glycosylation end product specific receptorAdvanced glycosylation end product-specific receptorAger
        DAMA 358M23.4LE 9211 A antigen MAPK MAK MRK overlapping kinase MGC22357MOKOTTHUMP00000174885RAGE 1RAGE_HUMANRAGE1Receptor for advanced glycation end productsReceptor for advanced glycosylation end productsRenal tumor antigen Renal tumor antigen 1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100632 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      RAGE Human ELISA Kit images

      • ELISA - RAGE Human ELISA Kit (ab100632)
        ELISA - RAGE Human ELISA Kit (ab100632)

      Protocols

      References for RAGE Human ELISA Kit (ab100632)

      ab100632 has not yet been referenced specifically in any publications.

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