RAGE Human ELISA Kit (ab100632)
- Product nameRAGE Human ELISA KitSee all RAGE kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 3 pg/ml
- Range2.06 pg/ml - 1500 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 103.4 94% - 112% Serum 89.25 79% - 98% Plasma 78.58 67% - 93%
- Species reactivityReacts with: Human
- Product overview
ab100632 RAGE Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human RAGE in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for human RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Optimization may be required with urine samples.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 200X HRP-Streptavidin Concentrate Item G 1 x 200µl 20X Wash Buffer Concentrate Item B 1 x 25ml Assay Diluent A diluent buffer . For Standard/Sample (serum/plasma) diluent. 1 x 30ml Assay Diluent B 5x concentrated buffer. For Standard/Sample (cell culture medium/urine) diluent. 1 x 15ml Detection Antibody RAGE : biotinylated anti-human RAGE 2 vials HRP-Streptavidin Concentrate 200x concentrated HRP-conjugated streptavidin 1 x 200µl Human RAGE Standard(recombinant) 2 vials RAGE Microplate (12 strips x 8 wells) coated with anti-human RAGE 1 unit
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Advanced glycosylation end product specific receptorAdvanced glycosylation end product-specific receptorAger
- DAMA 358M23.4LE 9211 A antigen MAPK MAK MRK overlapping kinase MGC22357MOKOTTHUMP00000174885RAGE 1RAGE_HUMANRAGE1Receptor for advanced glycation end productsReceptor for advanced glycosylation end productsRenal tumor antigen Renal tumor antigen 1
Our Abpromise guarantee covers the use of ab100632 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for RAGE Human ELISA Kit (ab100632)
ab100632 has not yet been referenced specifically in any publications.