You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameRAGE Mouse ELISA KitSee all RAGE kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich
  • Sensitivity
    < 15 pg/ml
  • Range
    13.72 pg/ml - 10000 pg/ml
  • Recovery

    87 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 75.89 67% - 82%
    Serum 102.9 91% - 110%
    Plasma 82.69 71% - 92%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Mouse
  • Product overview

    ab100738 RAGE Mouse ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of mouse RAGE in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for mouse RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-mouse RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsELISA more details
  • PlatformMicroplate

Properties

  • FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • Tissue specificityEndothelial cells.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.

    • Target information above from: UniProt accession Q15109 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
    • Alternative names
        Advanced glycosylation end product specific receptorAdvanced glycosylation end product-specific receptorAger
        DAMA 358M23.4LE 9211 A antigen MAPK MAK MRK overlapping kinase MGC22357MOKOTTHUMP00000174885RAGE 1RAGE_HUMANRAGE1Receptor for advanced glycation end productsReceptor for advanced glycosylation end productsRenal tumor antigen Renal tumor antigen 1
      see all
  • Database links

      • Applications

      Our Abpromise guarantee covers the use of ab100738 in the following tested applications.

      The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

      Application Notes
      ELISA ELISA

      RAGE Mouse ELISA Kit images

      • ELISA - RAGE Mouse ELISA Kit (ab100738)
        ELISA - RAGE Mouse ELISA Kit (ab100738)

      Protocols

      References for RAGE Mouse ELISA Kit (ab100738)

      ab100738 has not yet been referenced specifically in any publications.

      Publishing research using ab100738 ? Please let us know so that we can cite the reference in this datasheet

      Product Wall

      Submit an Abreview for ab100738 Submit a Question for ab100738

      Displaying 1 - 2 of 2 results for Abreviews and Q&A

      10
      Votes: Highest First

      Do you have a recommendation for dilution of mouse serum for this kit?

      Thank you for contacting us.

      The recommended dilution for testing normal mouse serum and plasma with this kit is 2-fold.

      I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or infor...

      Read More

      Our customer would like to purchase ab100738 and her samples are mouse muscle tissue.   Availible sample type describes "Cell culture supernatant, Serum, Plasma" on the datasheet and there's no product for tissue samples.   If the customer wants to use th...

      Read More

      Thank you for contacting us.  This ELISA kit has not been validated for use on tissue samples, but may be suitable for that purpose.  Theoretically, any low-salt lysis buffer that meets the following criteria should be compatible with this kit: 1)...

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"