RAGE Rat ELISA Kit (ab100780)
- Product nameRAGE Rat ELISA KitSee all RAGE kits ...
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Serum, Plasma
- Assay typeSandwich
- Sensitivity< 300 pg/ml
- Range0.41 ng/ml - 300 ng/ml
Sample specific recovery Sample type Average % Range Serum 79.4 70% - 91% Plasma 74.84 69% - 88%
- Species reactivityReacts with: Rat
- Product overview
ab100780 Rat RAGE (Receptor for Advanced Glycation End Products) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat RAGE in serum, plasma, cell culture supernatants and urine. This assay employs an antibody specific for Rat RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
- Tested applicationsELISA more details
- Storage instructionsStore at -20°C. Please refer to protocols.
Components Identifier 1 x 96 tests 20X Wash Buffer Concentrate Item B 1 x 25ml 400X HRP-Streptavidin Concentrate Item G 1 x 200µl Assay Diluent B 5x concentrated buffer. For detection antibody and HRP- Streptavidin diluent. 1 x 15ml Assay Diluent D 5x concentrated buffer. For Standard/Sample (serum/plasma samples/cell culture medium/urine) diluent. 1 x 15ml Detection Antibody RAGE: biotinylated anti-rat RAGE 2 vials RAGE Microplate (12 strips x 8 wells) coated with anti-rat RAGE 1 unit RAGE Standard Rat (recombinant) 2 vials Stop Solution Item I 1 x 8ml TMB One-Step Substrate Reagent Item H 1 x 12ml
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
- Advanced glycosylation end product specific receptorAdvanced glycosylation end product-specific receptorAger
- DAMA 358M23.4LE 9211 A antigen MAPK MAK MRK overlapping kinase MGC22357MOKOTTHUMP00000174885RAGE 1RAGE_HUMANRAGE1Receptor for advanced glycation end productsReceptor for advanced glycosylation end productsRenal tumor antigen Renal tumor antigen 1
Our Abpromise guarantee covers the use of ab100780 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
References for RAGE Rat ELISA Kit (ab100780)
ab100780 has not yet been referenced specifically in any publications.