Overview

  • Product nameRAGE Rat ELISA KitSee all RAGE kits ...
  • Detection methodColorimetric
  • Tests
    1 x 96 well plate
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay typeSandwich (quantitative)
  • Sensitivity
    < 300 pg/ml
  • Range
    0.41 ng/ml - 300 ng/ml
  • Recovery

    72 %

    Sample specific recovery
    Sample type Average % Range
    Serum 79.4 70% - 91%
    Plasma 74.84 69% - 88%
    Cell culture media 64.74 53% - 70%

  • Assay durationMultiple steps standard assay
  • Species reactivity
    Reacts with: Rat
  • Product overview

    Abcam’s RAGE Rat ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Rat RAGE in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Rat RAGE coated on a 96-well plate. Standards and samples are pipetted into the wells and RAGE present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Rat RAGE antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of RAGE bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applicationsSandwich ELISA more details
  • PlatformMicroplate

Properties

  • FunctionMediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
  • Tissue specificityEndothelial cells.
  • Sequence similaritiesContains 2 Ig-like C2-type (immunoglobulin-like) domains.
    Contains 1 Ig-like V-type (immunoglobulin-like) domain.
  • Cellular localizationSecreted and Cell membrane.
  • Target information above from: UniProt accession Q15109 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names
    • Advanced glycosylation end product specific receptor
    • Advanced glycosylation end product-specific receptor
    • Ager
    • DAMA 358M23.4
    • LE 9211 A antigen
    • MAPK MAK MRK overlapping kinase
    • MGC22357
    • MOK
    • OTTHUMP00000174885
    • RAGE 1
    • RAGE_HUMAN
    • RAGE1
    • Receptor for advanced glycation end products
    • Receptor for advanced glycosylation end products
    • Renal tumor antigen
    • Renal tumor antigen 1
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab100780 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Sandwich ELISA Use at an assay dependent concentration.

RAGE Rat ELISA Kit images

  • Representative standard curve using ab100780

Protocols

References for RAGE Rat ELISA Kit (ab100780)

ab100780 has not yet been referenced specifically in any publications.

Product Wall

The laboratory has informed me that ab100780 will detect soluble RAGE.

Thank you for contacting us.


Although this particular kit was neither designed nor validated with lysate samples, our kits are very accommodating to many different samples types so lysates will most likely work. If you decide to test th...

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Thank you for calling Abcam yesterday.

I have talked to the lab and we should be able to add the standard from ab100780 to the catalogue. It may take a week or so though. I will contact you with the abID when it is ready to be ordered.
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"