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Anti-RAGE antibody [DD/A11 or mAbA11] (ab78022)

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Overview

Product name

Anti-RAGE antibody [DD/A11 or mAbA11]
See all RAGE products (12) ...

Description

Mouse monoclonal [DD/A11 or mAbA11] to RAGE

Specificity

ab78022 recognizes both natural and recombinant RAGE.

Tested applications

WB, ELISA, IHC-P, ICCmore details

Cross reactivity

Reacts with

Mouse, Cow, Human

Predicted to work with

Rat

Immunogen

Recombinant fragment, corresponding to amino acids 90-347 (truncated extracellular portion)of Human MOK protein kinase

Positive control

Lung tissue; bovine lung extract; mouse brain lysate.

Properties

Form

Liquid

Storage instructions

Store at +4°C.

Storage buffer

Preservative: 0.1% Sodium Azide
Constituents: 0.25M Sodium chloride, 0.02M PBS

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Clone number

DD/A11 or mAbA11

Isotype

IgG2a

Research areas

Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cardiovascular >> Atherosclerosis >> Diabetes associated
Cancer >> Tumor biomarkers >> Tumor antigens
Cancer >> Tumor immunology >> Tumor-associated antigens
Neuroscience >> Sensory System >> Visual system
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> Other Kinases
Signal Transduction >> Protein Phosphorylation >> Ser / Thr Kinases >> MAPK Pathway
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid

  • Western blot - RAGE antibody [DD/A11 or mAbA11] (ab78022)Western blot - RAGE antibody [DD/A11 or mAbA11] (ab78022) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab78022 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
ICC (acetone-fixed): Use at an assay dependent dilution.
IHC-P: Use at an assay dependent dilution.
WB: 1/500. Detects a band of approximately 48 kDa (predicted molecular weight: 48 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.

Tissue specificity

Endothelial cells.

Sequence similarities

Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.

Cellular localization

Secreted and Cell membrane.

Target information above from: UniProt accessionQ15109 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Advanced glycosylation end product specific receptor antibody
  • Advanced glycosylation end product-specific receptor antibody
  • AGER antibody
  • EC 2.7.11.22 antibody
  • LE 9211 A antigen antibody
  • MAPK MAK MRK overlapping kinase antibody
  • MGC22357 antibody
  • MOK antibody
  • RAGE 1 antibody
  • RAGE_HUMAN antibody
  • RAGE1 antibody
  • Receptor for advanced glycation endproducts antibody
  • Receptor for advanced glycosylation end products antibody
  • Renal tumor antigen 1 antibody
  • Renal tumor antigen antibody
see all

Database links

see all

Anti-RAGE antibody [DD/A11 or mAbA11] images:

  Western blot - RAGE antibody [DD/A11 or mAbA11] (ab78022)

Western blot - RAGE antibody [DD/A11 or mAbA11] (ab78022)

Anti-RAGE antibody [DD/A11 or mAbA11] (ab78022) at 1/500 dilution + Mouse Brain lysate

Secondary
Goat anti-mouse HRP

Predicted band size : 48 kDa
Observed band size : 48 kDa

References for Anti-RAGE antibody [DD/A11 or mAbA11] (ab78022)

ab78022 has not yet been referenced specifically in any publications.

Publishing research using ab78022? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"