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Product name
Anti-RAGE antibody
See all RAGE products (12) ...
Description
Rabbit polyclonal to RAGE
Specificity
By Western blot, this antibody detects two bands in the 45 kDa range representing the RAGE protein pre and post glycosylation in mouse lung extract. This antibody also detects an ~25 kDa protein that is believed to be proteolytic degradation product. Immunohistochemical staining of RAGE in transgenic mouse retina results in staining of the retinal pigmented epithelium and photo receptor cell layers.
Tested applications
ICC/IF, IHC-Fr, WB, Flow Cyt, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide: (C)WRKRQPR(R/L)EERKAPESQED(NH2), corresponding to amino acids 362-380 of Rat RAGE
WRKRQPR(R/L)EERKAPESQED
Positive control
mouse lung extract
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
PBS, 1 mg/ml BSA and 0.05% sodium azide
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Sensory System >> Visual system
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
IHC-Fr: Use a concentration of 1 µg/ml.
WB: Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 42.6 kDa).
Flow Cyt: 1/100.
IHC-P: Use at an assay dependent dilution.
Target
Function
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
Tissue specificity
Endothelial cells.
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ15109
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Advanced glycosylation end product-specific receptor antibody
- AGER antibody
- LE-9211-A antigen antibody
see all
Database links
- Entrez Gene: 177 Human
- Entrez Gene: 11596 Mouse
- Entrez Gene: 81722 Rat
- Omim: 605762 Human
- SwissProt: Q15109 Human
- SwissProt: Q62151 Mouse
- SwissProt: Q63495 Rat
- Unigene: 104119 Human
see all
Anti-RAGE antibody images:
Western blot - RAGE antibody (ab3611)
Predicted band size : 42.6 kDa
ab3611 at a 2µg/ml concentration staining ~ 45 kDa RAGE in mouse lung lysate by Western blot (ECL).
Immunohistochemistry (Frozen sections) - RAGE antibody (ab3611)
Ab3611 used in IHC (frozen) in transgenic mouse retinas.
Immunocytochemistry/ Immunofluorescence - RAGE antibody (ab3611)
ab3611 at 1/200 staining mouse BV2 cells by ICC/IF. The cells were formaldehyde fixed, permeabilized and blocked with serum before incubation with the antibody for 1 hour. An Alexa Fluor ® 488 conjugated goat anti-rabbit antibody was used as the secondary. The left hand panel shows unstimulated cells, the right hand panel LPS stimulated cells.
This image is courtesy of an anonymous Abreview
References for Anti-RAGE antibody (ab3611)
This product has been referenced in:
- Deane R et al. A multimodal RAGE-specific inhibitor reduces amyloid ß-mediated brain disorder in a mouse model of Alzheimer disease. J Clin Invest 122:1377-92 (2012). Mouse.Read more (PubMed: 22406537) »
- Zhang L et al. S100B attenuates microglia activation in gliomas: possible role of STAT3 pathway. Glia 59:486-98 (2011). Flow Cyt, IHC-Fr; Mouse.Read more (PubMed: 21264954) »
See all 10 publications for this product
Publishing research using ab3611? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"