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Product name
Anti-RAGE antibody
See all RAGE products (12) ...
Description
Goat polyclonal to RAGE
Tested applications
IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Dog, Human
Immunogen
Synthetic peptide, corresponding to amino acids 42-59 of Human RAGE.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
serum with 0.01% sodium azide
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Sensory System >> Visual system
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Applications
Show applications key
Our Abpromise guarantee covers the use of ab7764 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use at an assay dependent dilution. PubMed: 16941014See Abreviews. Suggested working dilution of 1/400 from PMID 16941014.
Target
Function
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
Tissue specificity
Endothelial cells.
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ15109
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Advanced glycosylation end product-specific receptor antibody
- AGER antibody
- LE-9211-A antigen antibody
see all
Database links
- Entrez Gene: 403168 Dog
- Entrez Gene: 177 Human
- Entrez Gene: 11596 Mouse
- Omim: 605762 Human
- SwissProt: Q15109 Human
- SwissProt: Q62151 Mouse
- Unigene: 104119 Human
- Unigene: 534342 Human
see all
Anti-RAGE antibody images:
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAGE antibody (ab7764)
ab7764 at 1/100 staining mouse skin tissue sections by IHC-P. The tissue was paraformaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was blocked in serum and then incubated with the antibody. A Cy2 conjugated donkey anti-goat (F(ab')2 fragment) was used as the secondary antibody.
This image is courtesy of an Abreview submitted by Dr Carol Artlett
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAGE antibody (ab7764)
ab7764 staining RAGE in mouse lung tissue section by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent formaldehyde fixation before antigen retrieval which involved boiling citric acid and then blocking at 370C for 15 minutes was performed. The primary antibody was diluted in 1/250 and it was incubated with sample for 1 hour at 37°C. A HRP conjugated rat polyclonal to goat IgG was used undiluted as a secondary antibody.
This image is a courtesy of Anonymous Abreview
References for Anti-RAGE antibody (ab7764)
This product has been referenced in:
- Sterenczak KA et al. Quantitative PCR and immunohistochemical analyses of HMGB1 and RAGE expression in canine disseminated histiocytic sarcoma (malignant histiocytosis). Anticancer Res 31:1541-8 (2011). IHC-P; Dog.Read more (PubMed: 21617208) »
- Morbini P et al. The receptor for advanced glycation end products and its ligands: a new inflammatory pathway in lung disease? Mod Pathol 19:1437-45 (2006).Read more (PubMed: 16941014) »
See all 2 publications for this product
Publishing research using ab7764? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"