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- Secondary antibodies
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Product name
Anti-RAGE antibody
See all RAGE products (12) ...
Description
Rabbit polyclonal to RAGE
Tested applications
WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Cow, Dog, Pig
Immunogen
Synthetic peptide designed within aa 31-60 of Human RAGE protein (conjugated to KLH).
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Inflammatory mediators
Cardiovascular >> Atherosclerosis >> Diabetes associated
Neuroscience >> Sensory System >> Visual system
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Other
Neuroscience >> Neurology process >> Neurodegenerative disease >> Alzheimer's disease >> Amyloid
Applications
Show applications key
Our Abpromise guarantee covers the use of ab86767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/100 - 1/500. Detects a band of approximately 43 kDa (predicted molecular weight: 43 kDa).
IHC-P: 1/50 - 1/100.
Target
Function
Mediates interactions of advanced glycosylation end products (AGE). These are nonenzymatically glycosylated proteins which accumulate in vascular tissue in aging and at an accelerated rate in diabetes. Acts as a mediator of both acute and chronic vascular inflammation in conditions such as atherosclerosis and in particular as a complication of diabetes. AGE/RAGE signaling plays an important role in regulating the production/expression of TNF-alpha, oxidative stress, and endothelial dysfunction in type 2 diabetes. Interaction with S100A12 on endothelium, mononuclear phagocytes, and lymphocytes triggers cellular activation, with generation of key proinflammatory mediators. Interaction with S100B after myocardial infarction may play a role in myocyte apoptosis by activating ERK1/2 and p53/TP53 signaling (By similarity). Receptor for amyloid beta peptide. Contributes to the translocation of amyloid-beta peptide (ABPP) across the cell membrane from the extracellular to the intracellular space in cortical neurons. ABPP-initiated RAGE signaling, especially stimulation of p38 mitogen-activated protein kinase (MAPK), has the capacity to drive a transport system delivering ABPP as a complex with RAGE to the intraneuronal space.
Tissue specificity
Endothelial cells.
Sequence similarities
Contains 2 Ig-like C2-type (immunoglobulin-like) domains.
Contains 1 Ig-like V-type (immunoglobulin-like) domain.
Cellular localization
Secreted and Cell membrane.
Target information above from: UniProt accessionQ15109
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Advanced glycosylation end product specific receptor antibody
- Advanced glycosylation end product-specific receptor antibody
- AGER antibody
see all
Database links
- Entrez Gene: 280986 Cow
- Entrez Gene: 403168 Dog
- Entrez Gene: 177 Human
- Entrez Gene: 11596 Mouse
- Entrez Gene: 396591 Pig
- Omim: 605762 Human
- SwissProt: Q28173 Cow
- SwissProt: Q15109 Human
see all
Anti-RAGE antibody images:
Western blot - RAGE antibody (ab86767)
Anti-RAGE antibody (ab86767) at 1/500 dilution + Mouse lung whole cell lysate (35µg)
Secondary
Goat anti-rabbit IgG conjugated to HRP
Performed under reducing conditions.
Predicted band size : 43 kDa
Observed band size : 43 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RAGE antibody (ab86767)
ab86767 (1/100) staining RAGE in formalin fixed, paraffin embedded human lung carcinoma tissue. The secondary antibody was conjugated to HRP and the staining performed using the DAB method.
References for Anti-RAGE antibody (ab86767)
ab86767 has not yet been referenced specifically in any publications.
Publishing research using ab86767? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"