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Western blot - RALA+RALB antibody (ab42532)
Western blot - RALA+RALB antibody (ab42532)
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Product Name 

RALA+RALB antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to RALA+RALB

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RALA+RALB.

(Peptide available as ab42531.)

Reacts with 

Hu

Predicted to react 
(species key)

Ms, Rat, Cow, CynMk, Xl, Zfsh, Orngt
(Due to sequence homology)

Tested applications 
(see key)

WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 24 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

ab42532 gave a positive result in the following Whole Cell Lysates
HepG2
MDA MB 361

Cellular localization 

Cell Membrane

Research areas 

Chromatin and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> GTP

Relevance 

RALA, resorcylic acid lactone alpha, is a low molecular weight GTP binding protein belonging to the RAS family of onco proteins. RALA has a 50% amino acid homology with RAS and an 85% homology with RALB. GTP binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors.

 

Alternative names RALA+RALB antibody (ab42532)

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

5898    (Human)
5899    (Human)
56044    (Mouse)
81757    (Rat)
399386    (Xenopus laevis)

Omim

    

179551    (Human)
179550    (Human)

SwissProt

    

P11234    (Human)
P11233    (Human)
P05810    (Mouse)

Unigene

    

469820    (Human)
6906    (Human)
27348    (Mouse)
100380    (Rat)
663    (Xenopus laevis)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.500 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

 

At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RALA+RALB antibody is on this datasheet. But please do contact us if you would like any reassurance!


See below for RALA+RALB antibody images, references, products related to ab42532 and other tools.

 

RALA+RALB antibody images:

Product image  Western blot - RALA+RALB antibody (ab42532)

Western blot - RALA+RALB antibody (ab42532)

All lanes : RALA+RALB antibody (ab42532) at 1 µg/ml

Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 24 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)

 
 

Search PubMed (MEDLINE) for references to RALA+RALB

 

RALA+RALB antibody - more information

Customer reviews (feedback) regarding RALA+RALB antibody

Customer FAQs regarding RALA+RALB antibody

Protocols for RALA+RALB antibody

Price and availability of products related to RALA+RALB antibody

 

RALA+RALB antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Immunizing Peptide for RALA+RALB antibody

ab42531

  

RALA/RALB peptide

 

Compatible Secondaries for RALA+RALB antibody

ab6564

  

Cy5 ® antibody

ab6717

  

FITC antibody

ab6720

  

Biotin antibody

ab6721

  

HRP antibody

ab6722

  

Alkaline Phosphatase antibody

ab6793

  

Texas Red ® antibody

ab7007

  

Phycoerythrin antibody

ab7019

  

Agarose antibody

ab27234

  

10nm Gold antibody

ab27235

  

5nm Gold antibody

ab27236

  

15nm Gold antibody

ab27237

  

20nm Gold antibody

ab27238

  

30nm Gold antibody

ab27239

  

10nm Gold antibody

ab27240

  

5nm Gold antibody

ab31193

  

Duck polyclonal to Rabbit IgG

ab60314

  

Chromeo™ 488 antibody

ab60315

  

Chromeo™ 494 antibody

ab60317

  

Chromeo™ 546 antibody

ab60319

  

Chromeo™ 642 antibody

 

Other RALA+RALB Antibodies

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RALA+RALB antibody - other tools:

Contact Abcam with a Technical Enquiry about RALA+RALB antibody

 

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