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Anti-RALA+RALB antibody (ab42532)

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Overview

Product name

Anti-RALA+RALB antibody

Description

Rabbit polyclonal to RALA+RALB

Tested applications

WBmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Xenopus laevis, Zebrafish, Cynomolgus Monkey, Orangutan

Immunogen

Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human RALA+RALB.

Read Abcam's proprietary immunogen policy

(Peptide available as ab42531.)

Positive control

ab42532 gave a positive result in the following Whole Cell Lysates HepG2 MDA MB 361

Properties

Form

Liquid

Storage instructions

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Epigenetics and Nuclear Signaling >> Transcription >> Cancer susceptibility >> Proto-oncogenes
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> GTP

  • Western blot - RALA+RALB antibody (ab42532)Western blot - RALA+RALB antibody (ab42532) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab42532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

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    WB

     WB: Use a concentration ...Read more →

    WB: Use a concentration of 1 µg/mlDetects a band of approximately 27 kDa (predicted molecular weight: 24 kDa).

Target

Relevance

RALA, resorcylic acid lactone alpha, is a low molecular weight GTP binding protein belonging to the RAS family of onco proteins. RALA has a 50% amino acid homology with RAS and an 85% homology with RALB. GTP binding proteins mediate the transmembrane signaling initiated by the occupancy of certain cell surface receptors.

Cellular localization

Cell Membrane

Alternative names

  • Dm RalA antibody
  • DRal antibody
  • Drala antibody
  • Ral antibody
  • RalA protein antibody
  • Ras family small GTP binding protein RALA antibody
  • RAS like family 1 antibody
  • RAS like protein A antibody
  • RAS LIKE PROTEIN antibody
  • RAS like protein B antibody
  • Ras related protein antibody
  • Ras related protein RalA antibody
  • Rasl1 antibody
  • Vral simian leukemia viral oncogene homolog A (ras related) antibody
see all

Database links

see all

Anti-RALA+RALB antibody images:

  Western blot - RALA+RALB antibody (ab42532)

Western blot - RALA+RALB antibody (ab42532)

All lanes : Anti-RALA+RALB antibody (ab42532) at 1 µg/ml

Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate (ab7900)
Lane 2 : MDA MB 361 (Human breast adenocarcinoma cell line) Whole Cell Lysate

Lysates/proteins at 10 µg per lane.

Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

Performed under reducing conditions.

Predicted band size : 24 kDa
Observed band size : 27 kDa (why is the actual band size different from the predicted?)

References for Anti-RALA+RALB antibody (ab42532)

ab42532 has not yet been referenced specifically in any publications.

Publishing research using ab42532? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"