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Products:Signal Transduction >> Second Messenger >> Nucleotide Messenger >> GTP
Overview
Product name
Anti-RALGDS antibody
See all RALGDS products (3) ...
Description
Mouse polyclonal to RALGDS
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Full length RALGDS protein (Human)
Positive control
RALGDS transfected 293T cell lysate
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Regulators
Signal Transduction >> Second Messenger >> Nucleotide Messenger >> GTP
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Western blot - RALGDS antibody (ab68296)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab68296 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/500 - 1/1000.Detects a band of approximately 140 kDa (predicted molecular weight: 100 kDa).
Target
Relevance
Stimulates the dissociation of GDP from the Ras related RalA and RalB GTPases which allows GTP binding and activation of the GTPases. Interacts and acts as an effector molecule for R Ras, H Ras, K Ras, and Rap (from SwissProt).
Cellular localization
Cytoplasmic vesicle
Alternative names
- ral guanine nucleotide dissociation stimulator antibody
- RalGEF antibody
- RGF antibody
Database links
- Entrez Gene: 5900 Human
- Omim: 601619 Human
- SwissProt: Q12967 Human
Anti-RALGDS antibody images:
Western blot - RALGDS antibody (ab68296)
All lanes : Anti-RALGDS antibody (ab68296) at 1/500 dilution
Lane 1 : RALGDS transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 100 kDa
Observed band size : 140 kDa (why is the actual band size different from the predicted?)
References for Anti-RALGDS antibody (ab68296)
ab68296 has not yet been referenced specifically in any publications.
Publishing research using ab68296? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"