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Immunocytochemistry/ Immunofluorescence - RAMP3 antibody (ab58660)
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Product Name
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RAMP3 antibody
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See all RAMP3 antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RAMP3
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Immunogen
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A synthetic peptide corresponding to N-terminal residues of human RAMP3
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Reacts with
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Hu
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Tested applications
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ELISA, ICC/IF, WB
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Abreviews
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Application notes
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Recommended dilutions
ELISA: Use at an assay dependent dilution.
ICC/IF: Use at a concentration of 1 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 16 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cell Membrane; Single-pass type I membrane protein
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Research areas
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Signal Transduction >> Growth Factors/Hormones >> Hormones
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
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Relevance
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RAMP3 (receptor calcitonin activity modifying protein 3) transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. It acts as a receptor for adrenomedullin (AM) together with CALCRL. It is strongly expressed in lung, breast, immune system and fetal tissues and belongs to the RAMP family.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS Material safety datasheets (MSDS) for this product:
Glycerol MSDS
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RAMP3 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RAMP3 antibody images, references, products related to ab58660 and other tools.
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RAMP3 antibody images:
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 Immunocytochemistry/ Immunofluorescence - RAMP3 antibody (ab58660)
ICC/IF image of ab58660 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58660, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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Search PubMed (MEDLINE) for references to RAMP3
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RAMP3 antibody - more information
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Customer reviews (feedback) regarding RAMP3 antibody |
Customer FAQs regarding RAMP3 antibody |
Protocols for RAMP3 antibody |
Price and availability of products related to RAMP3 antibody |
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RAMP3 antibody - related products:
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RAMP3 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RAMP3 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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