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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
Overview
Product name
Anti-RAMP3 antibody
See all RAMP3 products (4) ...
Description
Rabbit polyclonal to RAMP3
Tested applications
ICC/IF, WB, ELISAmore details
Cross reactivity
Reacts with
Recombinant Fragment
Predicted to work with
Human
Immunogen
A synthetic peptide corresponding to N-terminal residues of human RAMP3
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.01% Sodium Azide
Constituents: 50% Glycerol, PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Growth Factors/Hormones >> Hormones
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> GPCR
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Immunocytochemistry/ Immunofluorescence - RAMP3 antibody (ab58660)
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Applications
Show applications key
Our Abpromise guarantee covers the use of ab58660 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 16 kDa.(This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.)
ELISA: Use at an assay dependent dilution.
Target
Relevance
RAMP3 (receptor calcitonin activity modifying protein 3) transports the calcitonin gene-related peptide type 1 receptor (CALCRL) to the plasma membrane. It acts as a receptor for adrenomedullin (AM) together with CALCRL. It is strongly expressed in lung, breast, immune system and fetal tissues and belongs to the RAMP family.
Cellular localization
Cell Membrane; Single-pass type I membrane protein
Alternative names
- Calcitonin receptor like receptor activity modifying protein 3 antibody
- CRLR activity modifying protein 3 antibody
- CRLR activity-modifying protein 3 antibody
see all
Database links
- Entrez Gene: 10268 Human
- Omim: 605155 Human
- SwissProt: O60896 Human
Anti-RAMP3 antibody images:
Immunocytochemistry/ Immunofluorescence - RAMP3 antibody (ab58660)
ICC/IF image of ab58660 stained HepG2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab58660, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-RAMP3 antibody (ab58660)
This product has been referenced in:
- Brekhman Vet al. Receptor activity modifying protein-3 mediates the protumorigenic activity of lysyl oxidase-like protein-2. FASEB J : (2010).Read more (PubMed: 20802105) »
See 1 publication for this product
Publishing research using ab58660? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"