Overview
- Product nameRANK Mouse ELISA Kit
- Tests1 x 96 well plate
- Sample typeCell culture supernatant, Tissue Extracts
- Assay typeSandwich
- Sensitivity< 2 pg/ml
- Range62.5 pg/ml - 4000 pg/ml
- Species reactivityReacts with: Mouse
- Product overview
ab119606 RANK Mouse ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse RANK specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse RANK amount of sample captured in plate.
No detectable cross-reactivity with any other cytokine.
- Notes
Store at 4°C for frequent use, at -20°C for infrequent use. Avoid multiple freeze-thaw cycles.
- Tested applicationsSandwich ELISA more details
Properties
- Storage instructionsStore at +4°C. Please refer to protocols.
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Components 1 x 96 tests 96-well plate precoated with anti-mouse RANK antibody (12 strips) 1 unit ABC diluent buffer 1 x 12ml Antibody diluent buffer 1 x 12ml Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti-mouse RANK antibody 1 x 130µl Lyophilized recombinant mouse RANK standard 2 vials Sample diluent buffer 1 x 30ml TMB color developing agent 1 x 10ml TMB stop solution 1 x 10ml -
Research Areas
Defects in TNFRSF11A are a cause of Paget disease of bone type 2 (PDB2) [MIM:602080]; also known as familial Paget disease of bone. PDB2 is a bone-remodeling disorder with clinical similarities to FEO. Unlike FEO, however, affected individuals have involvement of the axial skeleton with lesions in the spine, pelvis and skull.
Defects in TNFRSF11A are the cause of osteopetrosis autosomal recessive type 7 (OPTB7) [MIM:612301]; also called osteoclast-poor osteopetrosis with hypogammaglobulinemia. Osteopetrosis is a rare genetic disease characterized by abnormally dense bone, due to defective resorption of immature bone. The disorder occurs in two forms: a severe autosomal recessive form occurring in utero, infancy, or childhood, and a benign autosomal dominant form occurring in adolescence or adulthood. OPTB7 is characterized by paucity of osteoclasts, suggesting a molecular defect in osteoclast development. OPTB7 is associated with hypogammaglobulinemia.
Target information above from: UniProt accession
Q9Y6Q6
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CD265FEOLOH18CR1
- Loss of heterozygosity 18 chromosomal region 1ODFROFEOPTB7Osteoclast differentiation factor receptorOSTSPDB2Receptor activator of NF-KBReceptor activator of nuclear factor kappa BTNFRSF11ATNR11_HUMANTRANCERTumor necrosis factor receptor superfamily member 11ATumor necrosis factor receptor superfamily member 11a activator of NFKBTumor necrosis factor receptor superfamily member 11a NFKB activator
see all
- Entrez Gene: 21934 Mouse
- SwissProt: O35305 Mouse
Applications
Our Abpromise guarantee covers the use of ab119606 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| Sandwich ELISA | sELISA |
RANK Mouse ELISA Kit images
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Sandwich ELISA - RANK Mouse ELISA Kit (ab119606)Representative standard curve using ab119606.
Protocols
References for RANK Mouse ELISA Kit (ab119606)
ab119606 has not yet been referenced specifically in any publications.
