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- Secondary antibodies
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Product name
Anti-RANTES antibody [53405.111]
See all RANTES products (13) ...
Description
Rat monoclonal [53405.111] to RANTES
Tested applications
ELISA, Inhibition Assay, WBmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Recombinant full length protein (Mouse) expressed in E.coli.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Clonality
Monoclonal
Clone number
53405.111
Isotype
IgG2a
Research areas
Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokines (CC)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab10394 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use a concentration of 0.5 - 2 µg/ml.
Inhib: Use at an assay dependent dilution. This product can neutralize the biological activity of recombinant RANTES.
WB: Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 7.9 kDa.
Target
Function
Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.
Tissue specificity
T-cell and macrophage specific.
Sequence similarities
Belongs to the intercrine beta (chemokine CC) family.
Post-translational
modifications
N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.
Cellular localization
Secreted.
Target information above from: UniProt accessionP13501
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Beta chemokine RANTES precursor antibody
- CCL 5 antibody
- CCL5 antibody
see all
Database links
- Entrez Gene: 6352 Human
- Entrez Gene: 20304 Mouse
- Omim: 187011 Human
- SwissProt: P13501 Human
- SwissProt: P30882 Mouse
- Unigene: 489044 Human
- Unigene: 514821 Human
References for Anti-RANTES antibody [53405.111] (ab10394)
ab10394 has not yet been referenced specifically in any publications.
Publishing research using ab10394? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"