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Anti-RANTES antibody (ab10393)

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Overview

Product name

Anti-RANTES antibody
See all RANTES products (13) ...

Description

Goat polyclonal to RANTES

Specificity

In indirect ELISA and immunoblotting, this antibody does not cross-react with other chemokines including recombinant, human IL-8, recombinant, human GRO alpha, recombinant, human MIP1 alpha, recombinant, human MIP1 beta, recombinant, mouse MIP1 alpha, recombinant, mouse MIP1 beta and recombinant, human MCP1.

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant full length protein (Human) expressed in E. coli.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS, pH 7.4

Concentration

Concentration information loading...

Purity

Protein G purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Immunology >> Immune System Diseases >> Antiviral Signaling >> HIV-related
Cardiovascular >> Atherosclerosis >> Vascular Inflammation >> Leukocyte recruitment >> Chemokines
Immunology >> Innate Immunity >> Chemokines >> Beta Chemokines (CC)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab10393 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • ELISA

     

  • WB

     

Application notes

ELISA: 0.5 µg/ml detects 1 ng/well of recombinant, human RANTES.
WB: 1 µg/ml antibody detects 0.5 ng/lane of recombinant, human RANTES under non-reducing conditions. Predicted molecular weight: 11 kDa.

The ND50 of the antibody is defined as concentration of antibody resulting in a one-half maximal inhibition of bioactivity of recombinant, human RANTES, that ispresent at five times its own EC50 (the concentration of recombinant, human RANTES producing a one-half maximal bioactivity without antibody).

Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Chemoattractant for blood monocytes, memory T-helper cells and eosinophils. Causes the release of histamine from basophils and activates eosinophils. Binds to CCR1, CCR3, CCR4 and CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant RANTES protein induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form RANTES(3-68) acts as a natural chemotaxis inhibitor and is a more potent inhibitor of HIV-1-infection. The second processed form RANTES(4-68) exhibits reduced chemotactic and HIV-suppressive activity compared with RANTES(1-68) and RANTES(3-68) and is generated by an unidentified enzyme associated with monocytes and neutrophils.

Tissue specificity

T-cell and macrophage specific.

Sequence similarities

Belongs to the intercrine beta (chemokine CC) family.

Post-translational
modifications

N-terminal processed form RANTES(3-68) is produced by proteolytic cleavage, probably by DPP4, after secretion from peripheral blood leukocytes and cultured sarcoma cells.
The identity of the O-linked saccharides at Ser-27 and Ser-28 are not reported in PubMed:1380064. They are assigned by similarity.

Cellular localization

Secreted.

Target information above from: UniProt accessionP13501 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • Beta chemokine RANTES precursor antibody
  • CCL 5 antibody
  • CCL5 antibody
  • CCL5_HUMAN antibody
  • Chemokine (C C motif) ligand 5 antibody
  • Chemokine CC Motif Ligand 5 antibody
  • D17S136E antibody
  • EoCP antibody
  • Eosinophil chemotactic cytokine antibody
  • MGC17164 antibody
  • RANTES(4-68) antibody
  • Regulated upon activation normally T expressed and presumably secreted antibody
  • SCYA 5 antibody
  • SCYA5 antibody
  • SIS delta antibody
  • SIS-delta antibody
  • SISd antibody
  • Small inducible cytokine A5 (RANTES) antibody
  • Small inducible cytokine A5 antibody
  • Small inducible cytokine subfamily A (Cys Cys) member 5 antibody
  • Small-inducible cytokine A5 antibody
  • T cell specific protein p288 antibody
  • T cell specific RANTES protein antibody
  • T cell-specific protein P228 antibody
  • T-cell-specific protein RANTES antibody
  • TCP 228 antibody
  • TCP228 antibody
see all

Database links

References for Anti-RANTES antibody (ab10393)

ab10393 has not yet been referenced specifically in any publications.

Publishing research using ab10393? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"