RASGRP2 protein (Tagged-His Tag) (ab92130)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab92130 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- Calcium and DAG-regulatedCalcium and DAG-regulated guanine nucleotide exchange factor ICalcium-and diacylglycerol-regulated guanine nucleotide exchange factor I
- CalDAG-GEFICdc25 like proteinCdc25-like proteinF25B3.3 kinase like proteinF25B3.3 kinase-like proteinGRP2_HUMANHCDC25LRAS guanyl releasing protein 2RAS guanyl-releasing protein 2Rasgrp2
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.
modificationsIsoform 2 is palmitoylated and myristoylated while isoform 1.
RASGRP2 protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for RASGRP2 protein (Tagged-His Tag) (ab92130)
ab92130 has not yet been referenced specifically in any publications.