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Overview

  • Product nameRASGRP2 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 230-609 (380 amino acids) of Human RASGRP2 with an N terminal His tag. Observed mwt: 50 kDa (44 kDa predicted); UniProt ID = Q7LDG7.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 124 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesVAEKLLQLQNFNTLMAVVGGLSHSSISRLKETHSHVSPET IKLWEGLTELVTATGNYGN YRRRLAACVGFRFPILGVH LKDLVALQLALPDWLDPARTRLNGAKMKQLFSILEELAMV TSLRPPVQANPDLLSLLTVSLDQYQTEDELYQLSLQRE PRSKSSPTSPTSCTPPPRPPV LEEWTSAAKPKLDQALV VEHIEKMVESVFRNFDVDGDGHISQEEFQIIRGNFPYLSA FG DLDQNQDGCISREEMVSYFLRSSSVLGGRMGFVHNF QESNSLRPVACRHCKALILGIYK QGLKCRACGVNCHKQ CKDRLSVECRRRAQSVSLEGSAPSPSPMHSHHHRAFSFSL PRPG RRGSRPPEIREEEVQTVEDGVFDIHL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab92130 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        Calcium and DAG-regulatedCalcium and DAG-regulated guanine nucleotide exchange factor ICalcium-and diacylglycerol-regulated guanine nucleotide exchange factor I
        CalDAG-GEFICdc25 like proteinCdc25-like proteinF25B3.3 kinase like proteinF25B3.3 kinase-like proteinGRP2_HUMANHCDC25LRAS guanyl releasing protein 2RAS guanyl-releasing protein 2Rasgrp2
      see all
  • FunctionFunctions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.
  • Tissue specificityDetected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.
  • Sequence similaritiesBelongs to the RASGRP family.
    Contains 2 EF-hand domains.
    Contains 1 N-terminal Ras-GEF domain.
    Contains 1 phorbol-ester/DAG-type zinc finger.
    Contains 1 Ras-GEF domain.
  • Developmental stageExpressed in fetal brain, lung, liver and kidney.
  • DomainThe N-terminal Ras-GEF domain mediates association with F-actin.
  • Post-translational
    modifications    Isoform 2 is palmitoylated and myristoylated while isoform 1.
  • Cellular localizationCytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.

    • Target information above from: UniProt accession Q7LDG7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    RASGRP2 protein (Tagged-His Tag) images

    • SDS-PAGE - RASGRP2 protein (Tagged-His Tag) (ab92130)
      SDS-PAGE - RASGRP2 protein (Tagged-His Tag) (ab92130)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RASGRP2 protein (Tagged-His Tag) (ab92130)

    ab92130 has not yet been referenced specifically in any publications.

    Publishing research using ab92130 ? Please let us know so that we can cite the reference in this datasheet

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