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Anti-RAB1B antibody (ab76721)

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Overview

Product name

Anti-RAB1B antibody
See all RAB1B products (2) ...

Description

Mouse monoclonal to RAB1B

Tested applications

ELISA, WBmore details

Cross reactivity

Reacts with

Human

Immunogen

Recombinant fragment, corresponding to amino acids 91 - 202 of human RAB1B protein (NP_112243) with a tag

Positive control

A-431 cell lysate

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: PBS, pH 7.2

Concentration

Concentration information loading...

Purity

Protein A purified

Clonality

Monoclonal

Isotype

IgG2a

Light chain type

kappa

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family

  • Western blot - RAB1B antibody [Not required] (ab76721)Western blot - RAB1B antibody [Not required] (ab76721) image (enlarge)

  • Western blot - RAB1B antibody [Not required] (ab76721)Western blot - RAB1B antibody [Not required] (ab76721) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab76721 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution.
WB: Use at a concentration of 1 - 5 µg/ml. Predicted molecular weight: 22 kDa.

This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Function

Protein transport. Regulates vesicular transport between the endoplasmic reticulum and successive Golgi compartments.

Sequence similarities

Belongs to the small GTPase superfamily. Rab family.

Post-translational
modifications

Prenylated; by GGTase II, only after interaction of the substrate with Rab escort protein 1 (REP1).
AMPylation at Tyr-77 occurs in the switch 2 region and leads to moderate inactivation of the GTPase activity. It appears to prolong the lifetime of the GTP state of RAB1B by restricting access of GTPase effectors to switch 2 and blocking effector-stimulated GTP hydrolysis, thereby rendering RAB1B constitutively active.

Cellular localization

Membrane. Cytoplasm. Targeted by REP1 to membranes of specific subcellular compartments including endoplasmic reticulum, Golgi apparatus, and intermediate vesicles between these two compartments. In the GDP-form, colocalizes with GDI in the cytoplasm.

Target information above from: UniProt accessionQ9H0U4 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).

Information by UniProt

Alternative names

  • RAB1B antibody
  • RAB1B member RAS oncogene family antibody
  • RAB1B_HUMAN antibody
  • Ras related protein Rab1B antibody
  • Ras-related protein Rab-1B antibody
  • Small GTP binding protein antibody
see all

Database links

Anti-RAB1B antibody images:

  Western blot - RAB1B antibody [Not required] (ab76721)

Western blot - RAB1B antibody [Not required] (ab76721)

Anti-RAB1B antibody (ab76721) at 5 µg/ml + immunogen at 0.2 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

Predicted band size : 22 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)


Note: The Western blot is against a tagged recombinant protein fragment (the immunogen), the tag alone being 26 kDa in size.

  Western blot - RAB1B antibody [Not required] (ab76721)

Western blot - RAB1B antibody [Not required] (ab76721)

Anti-RAB1B antibody (ab76721) at 5 µg/ml + A431 cell lysate at 25 µg

Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution

Predicted band size : 22 kDa
Observed band size : 22 kDa

References for Anti-RAB1B antibody (ab76721)

ab76721 has not yet been referenced specifically in any publications.

Publishing research using ab76721? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"