Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Anti-RAB1B antibody (ab76721) at 5 µg/ml + immunogen at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
Predicted band size : 22 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Note: The Western blot is against a tagged recombinant protein fragment (the immunogen), the tag alone being 26 kDa in size.
Anti-RAB1B antibody (ab76721) at 5 µg/ml + A431 cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 22 kDa
Observed band size : 22 kDa
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