You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Related products

Reassurance, Refunds & Replacements

If your product does not perform as described on this datasheet, we will refund or replace your product...

Read our guarantee »
 

Anti-RAB40B antibody (ab82896)

CodeSizePriceAbpointsAvailability
    
Add to basket
 
  • -
  •   
  •   
  •   
  •  
  •   
  •   
Updating...

Overview

Product name

Anti-RAB40B antibody
See all RAB40B products (3) ...

Description

Rabbit polyclonal to RAB40B

Tested applications

WB, ELISAmore details

Cross reactivity

Reacts with

Human

Predicted to work with

Mouse, Rat, Cow, Zebrafish

Immunogen

Synthetic peptide corresponding to a region within internal sequence amino acids 144-193 (YAERLGVTFF EVSPLCNFNI TESFTELARI VLLRHGMDRL WRPSKVLSLQ) of Human RAB40B (NP_006813).

YAERLGVTFF EVSPLCNFNI TESFTELARI VLLRHGMDRL WRPSKVLSLQ

Run BLAST with BLAST the sequence with ExPASyRun BLAST with BLAST the sequence with NCBI

Positive control

293T cell lysate.

Properties

Form

Liquid

Storage instructions

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.

Storage buffer

Preservative: None
Constituents: 2% Sucrose, PBS

Concentration

Concentration information loading...

Purity

Immunogen affinity purified

Clonality

Polyclonal

Isotype

IgG

Research areas

Signal Transduction >> Protein Trafficking >> Vesicle Transport >> Regulation
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Cell Biology >> Proteolysis / Ubiquitin >> Proteasome / Ubiquitin >> Ubiquitin E2s and E3s >> SCF Complex E3 Ligase

  • Western blot - RAB40B antibody (ab82896)Western blot - RAB40B antibody (ab82896) image (enlarge)

Applications

Show applications key

Our Abpromise guarantee covers the use of ab82896 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application notes

ELISA: Use at an assay dependent dilution. Titre using peptide based assay: 1/12500.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 31 kDa.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Target

Relevance

RAB40B is similar to a yeast protein which suggests a role of the gene product in regulating secretory vesicles. It may be a substrate recognition component of a SCF like ECS (Elongin Cullin SOCS box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.

Cellular localization

Cell membrane; Lipid anchor; Cytoplasmic side

Alternative names

  • FLJ42385 antibody
  • SEC4L antibody
  • GTP binding protein homologous to Saccharomyces cerevisiae SEC4 antibody
  • RAB40B member RAS oncogene family antibody
  • RAR antibody
  • Rar protein antibody
  • Ras related protein Rab 40B antibody
  • RP23 293H17.2 antibody
  • SOCS box containing protein RAR antibody
see all

Database links

Anti-RAB40B antibody images:

  Western blot - RAB40B antibody (ab82896)

Western blot - RAB40B antibody (ab82896)

Anti-RAB40B antibody (ab82896) at 1 µg/ml (in 5% skim milk / PBS buffer) + 293T cell lysate at 10 µg

Secondary
HRP conjugated anti-Rabbit IgG at 1/50000 dilution

Predicted band size : 31 kDa
Observed band size : 38 kDa (why is the actual band size different from the predicted?)
Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Gel concentration: 12%

References for Anti-RAB40B antibody (ab82896)

ab82896 has not yet been referenced specifically in any publications.

Publishing research using ab82896? Please let us know so that we can cite the reference in this datasheet

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"