You have changed your country from  to  . Please be aware that this will change the currency in the purchasing process.

Overview

  • Product nameRBM10 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 306-852 (547 amino acids) of Human RBM10 with an N terminal His tag. Predicted MWt: 61 kDa; Uniprot ID = P98175 (isoform 4).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 105 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesGSKRDMASNEGSRISAASVASTAIAAAQWAISQASQGGEG TWATSEEPPVDYSY YQQDEGYGNSQGTESSLYAHGYLK GTKGPGITGTKGDPTGAGPEASLEPGADSV SMQAFSRA QPGAAPGIYQQSAEASSSQGTAANSQSYTIMSPAVLKSEL QSPTHP SSALPPATSPTAQESYSQYPVPDVSTYQYDET SGYYYDPQTGLYYDPNSQYYYN AQSQQYLYWDGERRTY VPALEQSADGHKETGAPSKEGKEKKEKHKTKTAQQIAK DMERWARSLNKQKENFKNSFQPISSLRDDERRESATADAG YAILEKKGALAERQ HTSMDLPKLASDDRPSPPRGLVAA YSGESDSEEEQERGGPEREEKLTDWQKLAC LLCRRQFP SKEALIRHQQLSGLHKQNLEIHRRAHLSENELEALEKNDM EQMKYR DRAAERREKYGIPEPPEPKRRKYGGISTASVD FEQPTRDGLGSDNIGSRMLQAM GWKEGSGLGRKKQGIV TPIEAQTRVRGSGLGARGSSYGVTSTESYKETLHKTMV TRFNEAQ
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91978 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DXS8237EG patch domain containing protein 9G patch domain-containing protein 9
        GPATC9GPATCH9HGNC9896KIAA0122MGC1132MGC997Rbm10RBM10_HUMANRNA binding motif protein 10RNA binding protein 10RNA binding protein S1-1RNA-binding motif protein 10RNA-binding protein 10RNA-binding protein S1-1S1-1TARPSZRANB5
      see all
  • FunctionMay be involved in post-transcriptional processing, most probably in mRNA splicing. Binds to RNA homopolymers, with a preference for poly(G) and poly(U) and little for poly(A).
  • Involvement in diseaseDefects in RBM10 are the cause of TARP syndrome (TARPS) [MIM:311900]. It is a disorder characterized by the Robin sequence (micrognathia, glossoptosis and cleft palate), talipes equinovarus and cardiac defects.
  • Sequence similaritiesContains 1 C2H2-type zinc finger.
    Contains 1 G-patch domain.
    Contains 1 RanBP2-type zinc finger.
    Contains 2 RRM (RNA recognition motif) domains.
  • Post-translational
    modifications    Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationNucleus. In the extranucleolar nucleoplasm constitutes hundreds of nuclear domains, which dynamically change their structures in a reversible manner. Upon globally reducing RNA polymerase II transcription, the nuclear bodies enlarge and decrease in number. They occur closely adjacent to nuclear speckles or IGCs (interchromatin granule clusters) but coincide with TIDRs.

    • Target information above from: UniProt accession P98175 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    RBM10 protein (Tagged-His Tag) images

    • SDS-PAGE - RBM10 protein (Tagged-His Tag) (ab91978)
      SDS-PAGE - RBM10 protein (Tagged-His Tag) (ab91978)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images colored control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RBM10 protein (Tagged-His Tag) (ab91978)

    ab91978 has not yet been referenced specifically in any publications.

    Publishing research using ab91978 ? Please let us know so that we can cite the reference in this datasheet

    Product Wall

    Submit an Abreview for ab91978 Submit a Question for ab91978

    There are currently no Abreviews or Questions for ab91978.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"