- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-RBM34 antibody
See all RBM34 primary antibodies -
DescriptionRabbit polyclonal to RBM34
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Host speciesRabbit
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Tested applicationsSuitable for: WB, IP, IHC-Pmore details
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Species reactivityReacts with: Human
Predicted to work with: Gorilla
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Immunogen
Synthetic peptide corresponding to a region between residues 370 and 430 of human RBM34 (CAI21936.1).
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Positive control
- HeLa whole cell lysate
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferPreservative: 0.09% Sodium Azide
Constituents: Tris citrate/phosphate buffer, pH 7-8 -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesab86787 was affinity purified using an epitope specific to RBM34 immobilized on solid support.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab86787 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 49 kDa. | |
| IP | Use at 10 µg/mg of lysate. | |
| IHC-P | 1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similaritiesBelongs to the RRM RBM34 family.
Contains 2 RRM (RNA recognition motif) domains. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localizationNucleus > nucleolus.
- Information by UniProt
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Database links
- Entrez Gene: 23029 Human
- SwissProt: P42696 Human
- Unigene: 535224 Human
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Alternative names
- KIAA0117 antibody
- Rbm34 antibody
- RBM34_HUMAN antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RBM34 antibody (ab86787)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling RBM34 with ab86787 at 1/1000 (1µg/ml). Detection: DAB. -
Western blot - RBM34 antibody (ab86787)All lanes : Anti-RBM34 antibody (ab86787) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 49 kDa
Observed band size: 49 kDa
Exposure time: 10 seconds -
Immunoprecipitation - RBM34 antibody (ab86787)Detection of RBM34 by Western Blot of Immunprecipitate.
ab86787 at 0.4 µg/ml staining RBM34 in HeLa whole cell lysate immunoprecipitated using ab86787 at 10 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 30 seconds.
References
ab86787 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"