Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555)
Key features and details
- Mouse monoclonal [23A7.C9] to RBM35A + RBM35B
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG1
Overview
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Product name
Anti-RBM35A + RBM35B antibody [23A7.C9] -
Description
Mouse monoclonal [23A7.C9] to RBM35A + RBM35B -
Host species
Mouse -
Specificity
ab106555 recognises both Mouse RBM35A and RBM35B. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Horse, Cow, Dog, Pig, Chimpanzee, Opossum -
Immunogen
Recombinant full length Mouse RBM35A with a proprietary tag (NP_918944.2).
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Positive control
- RBM35A or RBM35B transfected 293T cell extracts
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.88% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab106555 was purified from tissue culture supernatant by Protein A chromatography followed by extensive dialysis against the buffer stated. The antibody is sterile filtered. -
Clonality
Monoclonal -
Clone number
23A7.C9 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab106555 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Predicted molecular weight: 76, 77 kDa.
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Notes |
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WB
1/1000. Predicted molecular weight: 76, 77 kDa. |
Target
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Function
mRNA splicing factor that regulates the formation of epithelial cell-specific isoforms. Specifically regulates the expression of FGFR2-IIIb, an epithelial cell-specific isoform of FGFR2. Also regulates the splicing of CD44, CTNND1, ENAH, 3 transcripts that undergo changes in splicing during the epithelial-to-mesenchymal transition (EMT). Acts by directly binding specific sequences in mRNAs. Binds the GU-rich sequence motifs in the ISE/ISS-3, a cis-element regulatory region present in the mRNA of FGFR2. -
Tissue specificity
Epithelial cell-specific. Epithelial-specific expression in diverse tissues and organs with particularly notable levels of expression in skin and gastrointestinal epithelia. -
Sequence similarities
Belongs to the ESRP family.
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 454187 Chimpanzee
- Entrez Gene: 464289 Chimpanzee
- Entrez Gene: 510878 Cow
- Entrez Gene: 538640 Cow
- Entrez Gene: 487054 Dog
- Entrez Gene: 489745 Dog
- Entrez Gene: 100055214 Horse
- Entrez Gene: 54845 Human
see all -
Alternative names
- Epithelial splicing regulatory protein 1 antibody
- Epithelial splicing regulatory protein 2 antibody
- Esrp1 antibody
see all
Images
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All lanes : Anti-RBM35A + RBM35B antibody [23A7.C9] (ab106555) at 1/1000 dilution
Lane 1 : GFP transfected 293T cell extract
Lane 2 : RBM35A transfected 293T cell extract
Lane 3 : RBM35B transfected 293T cell extract
Lysates/proteins at 5 µg per lane.
Secondary
All lanes : anti-mouse ECL antibody at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 76, 77 kDa
Datasheets and documents
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Datasheet download
References (4)
ab106555 has been referenced in 4 publications.
- Chen KJ et al. Bleomycin-enhanced alternative splicing of fibroblast growth factor receptor 2 induces epithelial to mesenchymal transition in lung fibrosis. Biosci Rep 38:N/A (2018). PubMed: 30049844
- Jing J et al. Dynamic changes of urinary proteins in a rat model of acute hypercoagulable state induced by tranexamic acid. J Cell Physiol N/A:N/A (2018). PubMed: 30536986
- Chen L et al. Up-regulated miR-133a orchestrates epithelial-mesenchymal transition of airway epithelial cells. Sci Rep 8:15543 (2018). PubMed: 30341388
- Warzecha CC et al. An ESRP-regulated splicing programme is abrogated during the epithelial-mesenchymal transition. EMBO J 29:3286-300 (2010). PubMed: 20711167