Recombinant Anti-RBM47 antibody [EPR9658] (ab167164)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9658] to RBM47
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Human
Related conjugates and formulations
Overview
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Product name
Anti-RBM47 antibody [EPR9658]
See all RBM47 primary antibodies -
Description
Rabbit monoclonal [EPR9658] to RBM47 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt,ICC/IF or IP -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- A549, Caco-2, A431, NIH/3T3 and Raw264.7 cell lysates; Human breast carcinoma and colon tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR9658 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167164 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Predicted molecular weight: 64 kDa.
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IHC-P |
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 64 kDa. |
IHC-P
1/50 - 1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Sequence similarities
Belongs to the RRM RBM47 family.
Contains 3 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 54502 Human
- Entrez Gene: 245945 Mouse
- Entrez Gene: 305340 Rat
- SwissProt: A0AV96 Human
- SwissProt: Q91WT8 Mouse
- SwissProt: Q66H68 Rat
- Unigene: 518727 Human
- Unigene: 36863 Mouse
see all -
Alternative names
- NET18 antibody
- Rbm47 antibody
- Rbm47 RNA binding motif protein 47 antibody
see all
Images
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ab167164 showing +ve staining in Human normal uterus.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab167164 showing +ve staining in Human thyroid gland .
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab167164 showing +ve staining in Human prostate hyperplasia.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ab167164 showing +ve staining in Human lung carcinoma.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-RBM47 antibody [EPR9658] (ab167164) at 1/1000 dilution
Lane 1 : A549 cell lysate
Lane 2 : Caco-2 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lane 5 : RAW 264.7 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labeled goat anti-rabbit at 1/2000 dilution
Predicted band size: 64 kDa -
Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue labeling RBM47 with ab167164 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling RBM47 with ab167164 at 1/50 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (7)
ab167164 has been referenced in 7 publications.
- Dvir S et al. Uncovering the RNA-binding protein landscape in the pluripotency network of human embryonic stem cells. Cell Rep 35:109198 (2021). PubMed: 34077720
- Wang K et al. RNA-binding protein RBM47 stabilizes IFNAR1 mRNA to potentiate host antiviral activity. EMBO Rep 22:e52205 (2021). PubMed: 34160127
- Li Y et al. TNRC6 proteins modulate hepatitis C virus replication by spatially regulating the binding of miR-122/Ago2 complexes to viral RNA. Nucleic Acids Res N/A:N/A (2019). PubMed: 30997501
- Jiang QQ & Liu WB miR-25 Promotes Melanoma Progression by regulating RNA binding motif protein 47. Med Sci (Paris) 34 Focus issue F1:59-65 (2018). PubMed: 30403177
- Wang S et al. RNA Binding Proteins Control Transdifferentiation of Hepatic Stellate Cells into Myofibroblasts. Cell Physiol Biochem 48:1215-1229 (2018). PubMed: 30045014
- Rokavec M et al. Pan-cancer EMT-signature identifies RBM47 down-regulation during colorectal cancer progression. Sci Rep 7:4687 (2017). IHC-P ; Human . PubMed: 28680090
- Mobin MB et al. The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation. Nat Commun 7:12848 (2016). PubMed: 27665711