Anti-RBM5 antibody (ab69770)
Key features and details
- Rabbit polyclonal to RBM5
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RBM5 antibody
See all RBM5 primary antibodies -
Description
Rabbit polyclonal to RBM5 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse -
Immunogen
Synthetic peptide derived from an internal region of Human RBM5
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Positive control
- Brain tissue; extracts from HUVEC cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+ -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab69770 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa).
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IHC-P |
Use at an assay dependent concentration.
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 92 kDa (predicted molecular weight: 92 kDa). |
IHC-P
Use at an assay dependent concentration. |
Target
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Function
Component of the spliceosome A complex. Regulates alternative splicing of a number of mRNAs. May modulate splice site pairing after recruitment of the U1 and U2 snRNPs to the 5' and 3' splice sites of the intron. May both positively and negatively regulate aopotosis by regulating the alternative splicing of several genes involved in this process, including FAS and CASP2/caspase-2. In the case of FAS, promotes exclusion of exon 6 thereby producing a soluble form of FAS that inhibits apoptosis. In the case of CASP2/caspase-2, promotes exclusion of exon 9 thereby producing a catalytically active form of CASP2/Caspase-2 that induces apoptosis. -
Tissue specificity
Isoform 5 is widely expressed in normal tissues and is expressed at increased levels in T-leukemic cell lines. -
Sequence similarities
Belongs to the RBM5/RBM10 family.
Contains 1 C2H2-type zinc finger.
Contains 1 G-patch domain.
Contains 1 RanBP2-type zinc finger.
Contains 2 RRM (RNA recognition motif) domains. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 10181 Human
- Entrez Gene: 83486 Mouse
- Omim: 606884 Human
- SwissProt: P52756 Human
- SwissProt: Q91YE7 Mouse
- Unigene: 439480 Human
- Unigene: 259197 Mouse
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Alternative names
- FLJ39876 antibody
- G15 antibody
- H37 antibody
see all
Images
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Left: human brain tissue stained with ab69770 at 1/50 dilution; Right: the antibody was preincubated with the immunizing peptide.
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All lanes : Anti-RBM5 antibody (ab69770) at 1/500 dilution
Lane 1 : Extracts from HUVEC cells
Lane 2 : Extracts from HUVEC cells with immunizing peptide at 10 µg
Lysates/proteins at 15 µg per lane.
Predicted band size: 92 kDa
Observed band size: 92 kDa
Additional bands at: 31 kDa. We are unsure as to the identity of these extra bands.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab69770 has been referenced in 1 publication.
- Martínez FP & Tang Q Identification of cellular proteins that interact with human cytomegalovirus immediate-early protein 1 by protein array assay. Viruses 6:89-105 (2014). PubMed: 24385082