Anti-RBP1 antibody (ab154881)
Key features and details
- Rabbit polyclonal to RBP1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-RBP1 antibody
See all RBP1 primary antibodies -
Description
Rabbit polyclonal to RBP1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to a region within amino acids 10-103 of Human RBP1 (UniProt ID: P09455).
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Positive control
- NIH-3T3 and C2C12 whole cell lysates; H1229 cells; Human Colon tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154881 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 16 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
1/100 - 1/1000.
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Notes |
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WB
1/1000 - 1/10000. Predicted molecular weight: 16 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
1/100 - 1/1000. |
Target
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Function
Intracellular transport of retinol. -
Tissue specificity
Detected in nearly all the tissues with higher expression in adult ovary, pancreas, pituitary gland and adrenal gland, and fetal liver. -
Sequence similarities
Belongs to the calycin superfamily. Fatty-acid binding protein (FABP) family. -
Domain
Forms a beta-barrel structure that accommodates hydrophobic ligands in its interior. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 5947 Human
- Entrez Gene: 19659 Mouse
- Omim: 180260 Human
- SwissProt: P09455 Human
- SwissProt: Q00915 Mouse
- Unigene: 529571 Human
- Unigene: 279741 Mouse
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Alternative names
- Cellular retinol binding protein antibody
- Cellular retinol-binding protein antibody
- Cellular retinol-binding protein I antibody
see all
Images
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Anti-RBP1 antibody (ab154881) at 1/5000 dilution + H1299 whole cell lysate at 30 µg
Predicted band size: 16 kDa
15% SDS PAGE -
All lanes : Anti-RBP1 antibody (ab154881) at 1/5000 dilution
Lane 1 : NIH3T3 whole cell lysate
Lane 2 : C2C12 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 16 kDa
12 % SDS-PAGE -
Immunofluorescence analysis of methanol fixed H1299 cells, labeling RBP1 using ab154881 at 1/500 dilution. Lower image costained with Hoerchst 33342.
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Immunohistochemical analysis of paraffin embedded Human colon tissue, labeling RBP1 using ab154881 at 1/100 dilution.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (6)
ab154881 has been referenced in 6 publications.
- Zhao Y et al. WITHDRAWN: Circ_0000517 contributes to hepatocellular carcinoma progression by upregulating ARID4B via sponging miR-328-3p. Cell Signal N/A:109950 (2021). PubMed: 33582185
- Hoffmann C et al. Hepatic stellate cell hypertrophy is associated with metabolic liver fibrosis. Sci Rep 10:3850 (2020). PubMed: 32123215
- Sadler E et al. Cell-Specific Transcriptional Responses to Heat Shock in the Mouse Utricle Epithelium. Front Cell Neurosci 14:123 (2020). PubMed: 32528249
- Berner D et al. The protective variant rs7173049 at LOXL1 locus impacts on retinoic acid signaling pathway in pseudoexfoliation syndrome. Hum Mol Genet N/A:N/A (2019). PubMed: 30986821
- Gliniak CM et al. The retinol-binding protein receptor STRA6 regulates diurnal insulin responses. J Biol Chem 292:15080-15093 (2017). PubMed: 28733465
- Zheng Q et al. iTRAQ-Based Proteomic Analysis of Visual Cycle-Associated Proteins in RPE of rd12 Mice before and after RPE65 Gene Delivery. J Ophthalmol 2015:918473 (2015). WB . PubMed: 26124962