Anti-RCCD1 antibody (ab122667)
Key features and details
- Rabbit polyclonal to RCCD1
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RCCD1 antibody
See all RCCD1 primary antibodies -
Description
Rabbit polyclonal to RCCD1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
antigen sequence: PFIAVQPFPA LLDLPMGSDA VKASCGSRHT AVVTRTGELY TWGWGKYGQL GHEDTTSLDR PRRVEYFVDK QLQVKAVTCG PWNTYVYAVE KG, corresponding to amino acids 283-374 of Human RCCD1 .
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Positive control
- IHC-P: Human small intestine, skin, testis and fallopian tube tissues. ICC: A-431 cells. WB: HEK293T cell lysate - overexpressing RCCD1 with a C-terminal myc-DDK tag (~3.1kDa)
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab122667 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml.
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Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. |
Target
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Sequence similarities
Contains 4 RCC1 repeats. - Information by UniProt
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Database links
- Entrez Gene: 91433 Human
- SwissProt: A6NED2 Human
- Unigene: 655895 Human
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Alternative names
- MGC14386 antibody
- RCC1 domain-containing protein 1 antibody
- RCCD1 antibody
- RCCD1_HUMAN antibody
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human small intestine tissue labeling RCCD1 with ab122667 at 1/200.
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Lanes 2-3 : Anti-RCCD1 antibody (ab122667)
Lane 1 : Marker
Lane 2 : HEK293T cell lysate - vector only transfected
Lane 3 : HEK293T cell lysate - overexpressing RCCD1 with a C-terminal myc-DDK tag (~3.1kDa) -
ab122667, at 4 µg/ml staining RCCD1 in PFA/Triton X-100 fixed/permeabilized A431 cells by Immunofluorescence (green).
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human skin tissue labeling RCCD1 with ab122667 at 1/200.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human testis tissue labeling RCCD1 with ab122667 at 1/200.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human fallopian tube tissue labeling RCCD1 with ab122667 at 1/200.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab122667 has not yet been referenced specifically in any publications.