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Products:Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Overview
Product name
Anti-RECK antibody - Third spacer region
See all RECK products (7) ...
Description
Rabbit polyclonal to RECK - Third spacer region
Specificity
ab28269 recognises the third spacer region of RECK before GPI anchor domain.
Tested applications
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to the third spacer region of human RECK, before GPI anchor domain. (Peptide available as ab41255.)
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.05% Sodium Azide
Constituents: 50% Glycerol
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cell Biology >> Proteolysis / Ubiquitin >> Protease inhibitors >> Serine protease inhibitors >> RECK
Cancer >> Oncoproteins/suppressors >> Tumor suppressors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab28269 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB
Application notes
WB: 1/1000 when using colorimetric substrates such as BCIP/NBT - 1/5000 when using chemiluminescent substrates. Predicted molecular weight: 106 kDa. RECK is glycosylated and the apparent weight on SDS-PAGE gels is closer to 110 kDa. Dilution optimised using Chromogenic detection. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
Target
Function
Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression.
Tissue specificity
Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells.
Sequence similarities
Contains 3 Kazal-like domains.
Post-translational
modifications
N-glycosylated.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionO95980
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- hRECK antibody
- Membrane anchored glycoprotein (metastasis and invasion) antibody
- RECK antibody
see all
Database links
- Entrez Gene: 313488 Human
- Entrez Gene: 8434 Human
- Omim: 605227 Human
- SwissProt: O95980 Human
- SwissProt: Q5W0K6 Human
- Unigene: 388918 Human
- Unigene: 728961 Human
Protocols
References for Anti-RECK antibody - Third spacer region (ab28269)
ab28269 has not yet been referenced specifically in any publications.
Publishing research using ab28269? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"