Anti-RECK antibody (ab88249)
Key features and details
- Mouse polyclonal to RECK
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RECK antibody
See all RECK primary antibodies -
Description
Mouse polyclonal to RECK -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human RECK. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- RECK transfected 293T cell line and RECK expression in 293 cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Constituent: 100% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab88249 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 106 kDa. |
Target
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Function
Negatively regulates matrix metalloproteinase-9 (MMP-9) by suppressing MMP-9 secretion and by direct inhibition of its enzymatic activity. RECK down-regulation by oncogenic signals may facilitate tumor invasion and metastasis. Appears to also regulate MMP-2 and MT1-MMP, which are involved in cancer progression. -
Tissue specificity
Expressed in various tissues and untransformed cells. It is undetectable in tumor-derived cell lines and oncogenically transformed cells. -
Sequence similarities
Contains 3 Kazal-like domains. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 313488 Human
- Entrez Gene: 8434 Human
- Omim: 605227 Human
- SwissProt: O95980 Human
- SwissProt: Q5W0K6 Human
- Unigene: 728961 Human
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Alternative names
- hRECK antibody
- Membrane anchored glycoprotein (metastasis and invasion) antibody
- RECK antibody
see all
Images
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Anti-RECK antibody (ab88249) at 1 µg/ml + 293 cell lysate at 50 µg
Predicted band size: 106 kDa -
All lanes : Anti-RECK antibody (ab88249) at 1 µg/ml
Lane 1 : RECK transfected 293T cell line
Lane 2 : Non-transfected 293T cell line
Lysates/proteins at 25 µg per lane.
Predicted band size: 106 kDa
Observed band size: 106 kDa
Datasheets and documents
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Datasheet download
References (5)
ab88249 has been referenced in 5 publications.
- Abdelkader NF et al. Empagliflozin mitigates type 2 diabetes-associated peripheral neuropathy: a glucose-independent effect through AMPK signaling. Arch Pharm Res 45:475-493 (2022). PubMed: 35767208
- Wei ZL et al. MicroRNA-221 promotes papillary thyroid carcinoma cell migration and invasion via targeting RECK and regulating epithelial-mesenchymal transition. Onco Targets Ther 12:2323-2333 (2019). PubMed: 30992669
- Iseki Y et al. MicroRNA-96 Promotes Tumor Invasion in Colorectal Cancer via RECK. Anticancer Res 38:2031-2035 (2018). PubMed: 29599320
- Liu W et al. miR-221 and miR-222 Simultaneously Target RECK and Regulate Growth and Invasion of Gastric Cancer Cells. Med Sci Monit 21:2718-25 (2015). WB ; Human . PubMed: 26364844
- Zhou XQ et al. Epigallocatechin-3-gallate inhibits the invasion of salivary adenoid cystic carcinoma cells by reversing the hypermethylation status of the RECK gene. Mol Med Rep 12:6031-6 (2015). PubMed: 26299812