Anti-RECQL4 antibody (ab188125)
Key features and details
- Rabbit polyclonal to RECQL4
- Suitable for: IHC-P, ICC/IF, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RECQL4 antibody
See all RECQL4 primary antibodies -
Description
Rabbit polyclonal to RECQL4 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, ICC/IF, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human RECQL4 aa 150-350.
Database link: O94761 -
Positive control
- IHC-P: Human thyroid gland tissue. ICC/IF: A431 cells. WB: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab188125 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 133 kDa.
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Notes |
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IHC-P
1/20 - 1/50. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 133 kDa. |
Target
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Function
DNA-dependent ATPase. May modulate chromosome segregation. -
Tissue specificity
Ubiquitously expressed, with highest levels in thymus and testis. -
Involvement in disease
Defects in RECQL4 are a cause of Rothmund-Thomson syndrome (RTS) [MIM:268400]. A disease characterized by dermatological features such as atrophy, pigmentation, and telangiectasia and frequently accompanied by juvenile cataract, saddle nose, congenital bone defects, disturbances of hair growth, and hypogonadism.
Defects in RECQL4 are a cause of RAPADILINO syndrome (RAPADILINOS) [MIM:266280]. A disease characterized by radial and patellar aplasia or hypoplasia.
Defects in RECQL4 are a cause of Baller-Gerold syndrome (BGS) [MIM:218600]; also known as craniosynostosis with radial defects. BGS is an autosomal recessive syndrome characterized by short stature, craniosynostosis, absent or hypoplastic radii, short and curved ulna, fused carpal bones and absent carpals, metacarpals and phalanges. Some patients manifest poikiloderma. Cases reported as Baller-Gerold syndrome have phenotypic overlap with several other disorders, including Saethre-Chotzen syndrome. BGS is part of the clinical spectrum of Rothmund-Thomson and RAPADILINO syndromes. -
Sequence similarities
Belongs to the helicase family. RecQ subfamily.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 9401 Human
- Omim: 603780 Human
- SwissProt: O94761 Human
- Unigene: 31442 Human
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Alternative names
- ATP dependent DNA helicase Q4 antibody
- ATP-dependent DNA helicase Q4 antibody
- DNA helicase antibody
see all
Images
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Anti-RECQL4 antibody (ab188125) at 0.4 µg/ml + U-2 OS cells
Predicted band size: 133 kDa -
Paraffin embedded human thyroid gland tissue stained for RECQL4 using ab188125 at 1/50 dilution in immunohistochemical analysis.
Heat mediated antigen retrieval was performed with citrate buffer pH 6 before the IHC staining protocol.
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Immunocytochemistry/ Immunofluorescence staining of human cell line A431 staining RECQL4 with ab188125. Antibody staining is shown in green.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab188125 has been referenced in 1 publication.
- Anwar T et al. Next-generation sequencing identifies recurrent copy number variations in invasive breast carcinomas from Ghana. Mod Pathol 33:1537-1545 (2020). PubMed: 32152520