Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
Overview
Product name
Anti-RECQL5 antibody
See all RECQL5 products (4) ...
Description
Rabbit polyclonal to RECQL5
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Rat, Dog
Immunogen
Synthetic peptide derived from within residues 950 to the C-terminus of Human RECQL5.
(Peptide available as ab31608.)
Positive control
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate;
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Primary antibody notes
This antibody appears to recognise the beta isoform of RecQL5 (109 kDa)
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
-
Western blot - RECQL5 antibody (ab31609)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31609 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 - 5 µg/ml.Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).
Target
Relevance
RecQ helicases are implicated in DNA recombination and repair helicases and are critical in genome maintenance. RecQL5 is one of five known RecQ homologues in humans, and defects in three of these (BLM, WRN and RecQ4)are associated with autosomal recessive disorders that involve genomic instability and a predisposition to cancer. The exact role of ReqL5 is unclear. It has been shown in mouse embryonic stem (ES) cells that mutations in the Recql5 genes result in a significant increase in the frequency of sister chromatid exchange (SCE), whereas deleting Recql5 lead to an even higher frequency of SCE. There are three different RecQL5 isoforms - alpha, beta and gamma, produced by alternative splicing.
Cellular localization
Cytoplasmic and Nuclear, depending on the isoform.
Alternative names
- ATP-dependent DNA helicase Q5 antibody
- DNA helicase, RECQ-like, type 5 antibody
- EC 3.6.1.- antibody
see all
Database links
- Entrez Gene: 9400 Human
- Entrez Gene: 287834 Rat
- Omim: 603781 Human
- SwissProt: O94762 Human
- SwissProt: Q6FIC9 Human
- SwissProt: Q6P4G0 Human
- Unigene: 632229 Human
- Unigene: 198916 Rat
Anti-RECQL5 antibody images:
Western blot - RECQL5 antibody (ab31609)
All lanes : Anti-RECQL5 antibody (ab31609) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 109 kDa
Observed band size : 109 kDa
Additional bands at : >300 kDa (possible cross reactivity).
References for Anti-RECQL5 antibody (ab31609)
This product has been referenced in:
- Blundred Ret al. Human RECQL5 overcomes thymidine-induced replication stress. DNA Repair (Amst) 9:964-75 (2010).Read more (PubMed: 20643585) »
See 1 publication for this product
Publishing research using ab31609? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"