|
|
|
|
Western blot - RECQL5 antibody (ab31609)
(enlarge)
|
|
- RECQL5 antibody (ab31609)
(enlarge)
|
|
|
|
|
Product Name
|
RECQL5 antibody
|
|
Product type
|
Primary antibodies
|
|
Description
|
Rabbit polyclonal to RECQL5
|
|
Immunogen
|
Synthetic peptide derived from within residues 950 to the C-terminus of Human RECQL5.
(Peptide available as ab31608.)
|
|
Reacts with
|
Hu
|
|
Predicted to react
(species key)
|
Rat, Dog
(Due to sequence homology)
|
|
Tested applications
(see key)
|
WB
|
|
|
Abreviews
|
|
|
|
Application notes
(see key)
|
Recommended dilutions
WB: Use at a concentration of 1 - 5 µg/ml. Detects a band of approximately 109 kDa (predicted molecular weight: 109 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
|
|
Positive control
(see definition)
|
HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate ; Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate;
|
|
Cellular localization
|
Cytoplasmic and Nuclear, depending on the isoform.
|
|
Research areas
|
Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Damage & Repair >> DNA Damage Response >> Other
|
|
Relevance
|
RecQ helicases are implicated in DNA recombination and repair helicases and are critical in genome maintenance. RecQL5 is one of five known RecQ homologues in humans, and defects in three of these (BLM, WRN and RecQ4)are associated with autosomal recessive disorders that involve genomic instability and a predisposition to cancer. The exact role of ReqL5 is unclear. It has been shown in mouse embryonic stem (ES) cells that mutations in the Recql5 genes result in a significant increase in the frequency of sister chromatid exchange (SCE), whereas deleting Recql5 lead to an even higher frequency of SCE. There are three different RecQL5 isoforms - alpha, beta and gamma, produced by alternative splicing.
This antibody appears to recognise the beta isoform of RecQL5 (109 kDa)
|
|
|
|
|
Database links
|
The links below go to external sites and will open in a new browser window |
|
Raised in
|
Rabbit
|
|
Clonality
|
Polyclonal
|
|
Isotype
|
IgG
|
|
Purity
|
Immunogen affinity purified
|
|
Storage buffer
|
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
|
|
Form
|
Liquid
|
|
Concentration
|
1.000 mg/ml
|
|
Storage instructions
|
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
|
| |
At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RECQL5 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
 |
|
|
See below for RECQL5 antibody images, references, products related to ab31609 and other tools.
|
| |
RECQL5 antibody images:
|
 Western blot - RECQL5 antibody (ab31609)
All lanes : RECQL5 antibody (ab31609) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate (ab7899)
Lysates/proteins at 20 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 109 kDa
Observed band size : 109 kDa
Additional bands at : >300 kDa (possible cross reactivity).
|
| |
- RECQL5 antibody (ab31609)
ICC/IF image of ab31609 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab31609, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2 and MCF7 cells at 5µg/ml.
|
| |
| |
|
Search PubMed (MEDLINE) for references to RECQL5
|
| |
RECQL5 antibody - more information
|
Customer reviews (feedback) regarding RECQL5 antibody |
Customer FAQs regarding RECQL5 antibody |
Protocols for RECQL5 antibody |
Price and availability of products related to RECQL5 antibody |
| |
RECQL5 antibody - related products:
|
|
|
| |
RECQL5 antibody - other tools:
|
Contact Abcam with a Technical Enquiry about RECQL5 antibody |
| |
All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
