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RENT1/hUPF1 protein (Tagged-His Tag) (ab91954)

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Overview

  • Product nameRENT1/hUPF1 protein (Tagged-His Tag)
  • Protein descriptionRecombinant fragment, corresponding to amino acids 703-1118 of Human RENT1/hUPF1 with N terminal His tag; 416 amino acids, 67 kDa (predicted MW 46 kDa); UniProt ID: Q92900_2.
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag.
  • FormLyophilised:Reconstitute with 69 µl distilled water.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Additional notesProtein Identity confirmed by Mass Spectrometry (MS/MS) (acquired on initial reference batch).
  • Sequence notesRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQP DKPMFFYVTQGQ EEIASSGTSYLNRTEAANVEKITTKL LKAGAKPDQIGIITPYEGQRSYLVQY MQFSGSLHTKLY QEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRR LNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQK VLVEGPLNNLRESL MQFSKPRKLVNTINPGARFMTTAM YDAREAIIPGSVYDRSSQGRPSSMYFQT HDQIGMISAG PSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPK GK TGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFS QGALTQGYISMSQPSQ MSQPGLSQPELSQDSYLGDEFK SQIDVALSQDSTYQGERAYQHGGVTGLSQY
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91954 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    MS
    SDS-PAGE
  • Application notesMS: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ATP dependent helicase RENT1ATP-dependent helicase RENT1Delta helicase
        FLJ43809FLJ46894HUPF 1HUPF1KIAA0221Nonsense mRNA reducing factor 1NORF 1NORF1pNORF 1pNORF1Regulator of nonsense transcripts 1RENT 1RENT1RENT1_HUMANSmg 2Smg 2 homolog nonsense mediated mRNA decay factorUP Frameshift 1Up frameshift mutation 1 homologUp frameshift mutation 1 homolog (S. cerevisiae)Up frameshift suppressor 1 homologUp-frameshift suppressor 1 homologUPF 1UPF 1 regulator of nonsense transcripts homologUPF1UPF1 regulator of nonsense transcripts homologYeast Upf1p homolog
      see all
  • FunctionPlays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
  • Tissue specificityUbiquitous.
  • Sequence similaritiesBelongs to the DNA2/NAM7 helicase family.
    Contains 1 C2H2-type zinc finger.
  • DomainThe [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
  • Post-translational
    modifications    Phosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
  • Cellular localizationCytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.

    • Target information above from: UniProt accession Q92900 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    RENT1/hUPF1 protein (Tagged-His Tag) images

    • SDS-PAGE - RENT1/hUPF1 protein (Tagged-His Tag) (ab91954)
      SDS-PAGE - RENT1/hUPF1 protein (Tagged-His Tag) (ab91954)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RENT1/hUPF1 protein (Tagged-His Tag) (ab91954)

    ab91954 has not yet been referenced specifically in any publications.

    Publishing research using ab91954 ? Please let us know so that we can cite the reference in this datasheet

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