RENT1/hUPF1 protein (Tagged-His Tag) (ab91954)
This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag.
Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
Our Abpromise guarantee covers the use of ab91954 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
- ATP dependent helicase RENT1ATP-dependent helicase RENT1Delta helicase
- FLJ43809FLJ46894HUPF 1HUPF1KIAA0221Nonsense mRNA reducing factor 1NORF 1NORF1pNORF 1pNORF1Regulator of nonsense transcripts 1RENT 1RENT1RENT1_HUMANSmg 2Smg 2 homolog nonsense mediated mRNA decay factorUP Frameshift 1Up frameshift mutation 1 homologUp frameshift mutation 1 homolog (S. cerevisiae)Up frameshift suppressor 1 homologUp-frameshift suppressor 1 homologUPF 1UPF 1 regulator of nonsense transcripts homologUPF1UPF1 regulator of nonsense transcripts homologYeast Upf1p homolog
Contains 1 C2H2-type zinc finger.
modificationsPhosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
RENT1/hUPF1 protein (Tagged-His Tag) images
The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.
References for RENT1/hUPF1 protein (Tagged-His Tag) (ab91954)
ab91954 has not yet been referenced specifically in any publications.