Anti-RENT1/hUPF1 antibody [EPR4681] (ab109363)

Overview

• Product nameAnti-RENT1/hUPF1 antibody [EPR4681]See all RENT1/hUPF1 primary antibodies ...
• Description
  Rabbit monoclonal [EPR4681] to RENT1/hUPF1
• Tested applicationsWB, IP, IHC-P, Flow Cyt, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  

  Does not react with

  Rat
• Immunogen

  Synthetic peptide, corresponding to residues in human RENT1/hUPF1.

• Positive controlHuT-78, Raji, SH-SY5Y and HeLa cell lysates, Human kidney tissue, Human transitional cell carcinoma tissue, HeLa cells.
• General notesProduced under U.S. Patent No. 5,675,063

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR4681
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • RNA Processing
  • Other

Applications

Target

• FunctionPlays a role in replication-dependent histone mRNA degradation at the end of phase S. Part of a post-splicing multiprotein complex. Involved in nonsense-mediated decay (NMD) as part of the SMG1C complex, a mRNA surveillance complex that recognizes and degrades mRNAs containing premature translation termination codons (PTCs). The complex probably acts by associating with ribosomes during tranlation termination on mRNPs. If an exon junction complex (EJC) is located 50-55 or more nucleotides downstream from the termination codon, RENT1 is phosphorylated by SMG1, triggering nonsense-mediated decay (NMD). Essential for embryonic viability.
• Tissue specificityUbiquitous.
• Sequence similaritiesBelongs to the DNA2/NAM7 helicase family.
  Contains 1 C2H2-type zinc finger.
• DomainThe [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.
• Post-translational
  modificationsPhosphorylated by SMG1; required for formation of mRNA surveillance complexes. Phosphorylated upon DNA damage, probably by ATM or ATR.
• Cellular localizationCytoplasm. Cytoplasm > P-body. Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm.

Target information above from: UniProt accession Q92900 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5976 Human
  • Entrez Gene: 19704 Mouse
  • Omim: 601430 Human
  • SwissProt: Q92900 Human
  • SwissProt: Q9EPU0 Mouse
  • Unigene: 515266 Human
  • Unigene: 390048 Mouse

• Alternative names
  ATP dependent helicase RENT1 antibodyATP-dependent helicase RENT1 antibodyDelta helicase antibody

  FLJ43809 antibodyFLJ46894 antibodyHUPF 1 antibodyhUpf1 antibodyKIAA0221 antibodyNonsense mRNA reducing factor 1 antibodyNORF 1 antibodyNORF1 antibodypNORF 1 antibodypNORF1 antibodyRegulator of nonsense transcripts 1 antibodyRENT 1 antibodyRENT1 antibodyRENT1_HUMAN antibodySmg 2 antibodySmg 2 homolog nonsense mediated mRNA decay factor antibodyUP Frameshift 1 antibodyUp frameshift mutation 1 homolog (S. cerevisiae) antibodyUp frameshift mutation 1 homolog antibodyUp frameshift suppressor 1 homolog antibodyUp-frameshift suppressor 1 homolog antibodyUPF 1 antibodyUPF 1 regulator of nonsense transcripts homolog antibodyupf1 antibodyUPF1 regulator of nonsense transcripts homolog antibodyYeast Upf1p homolog antibody

  see all

Anti-RENT1/hUPF1 antibody [EPR4681] images

• 

  Western blot - RENT1/hUPF1 antibody [EPR4681] (ab109363)
  All lanes : Anti-RENT1/hUPF1 antibody [EPR4681] (ab109363) at 1/10000 dilution
  
  Lane 1 : HuT-78 cell lysate
  Lane 2 : Raji cell lysate
  Lane 3 : SH-SY5Y cell lysate
  Lane 4 : HeLa cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  
  Performed under reducing conditions.
  
  Predicted band size : 124 kDa
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RENT1/hUPF1 antibody [EPR4681] (ab109363)
  ab109363, at 1/100, staining RENT1/hUPF1 in Human kidney tissue by immunohistochemistry.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RENT1/hUPF1 antibody [EPR4681] (ab109363)
  ab109363, at 1/100, staining RENT1/hUPF1 in Human transitional cell carcinoma tissue by immunohistochemistry.
• 

  Immunocytochemistry/ Immunofluorescence - RENT1/hUPF1 antibody [EPR4681] (ab109363)
  ab109363, at 1/100, staining RENT1/hUPF1 in HeLa cells by immunofluorescence.
• 

  Flow Cytometry-Anti-RENT1/hUPF1 antibody [EPR4681](ab109363)
  Overlay histogram showing HeLa cells stained with ab109363 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109363, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.