Anti-REXO2 antibody (ab206694)
Key features and details
- Rabbit polyclonal to REXO2
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-REXO2 antibody
See all REXO2 primary antibodies -
Description
Rabbit polyclonal to REXO2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Sheep, Horse, Cow, Cat, Dog, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide within Human REXO2 aa 150 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: Q9Y3B8 -
Positive control
- HeLa, 293T and Jurkat whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab206694 was affinity purified using an epitope specific to REXO2 immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab206694 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 27 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 27 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
3'-to-5' exoribonuclease specific for small oligoribonucleotides. Active on small (primarily -
Sequence similarities
Belongs to the oligoribonuclease family.
Contains 1 exonuclease domain. -
Cellular localization
Mitochondrion and Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 101083700 Cat
- Entrez Gene: 451561 Chimpanzee
- Entrez Gene: 540139 Cow
- Entrez Gene: 102130135 Cynomolgus monkey
- Entrez Gene: 489395 Dog
- Entrez Gene: 101126113 Gorilla
- Entrez Gene: 100062339 Horse
- Entrez Gene: 25996 Human
see all -
Alternative names
- 1810038D15Rik antibody
- AW107347 antibody
- CGI 114 antibody
see all
Images
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All lanes : Anti-REXO2 antibody (ab206694) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 27 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Detection of REXO2 in immunoprecipitates of 293T whole cell lysates prepared using NETN lysis buffer (1 mg for IP, 20% of IP loaded) using ab206694 at 6 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP.
Detection: Chemiluminescence with an exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab206694 has been referenced in 2 publications.
- Szewczyk M et al. Human REXO2 controls short mitochondrial RNAs generated by mtRNA processing and decay machinery to prevent accumulation of double-stranded RNA. Nucleic Acids Res 48:5572-5590 (2020). PubMed: 32365187
- Nicholls TJ et al. Dinucleotide Degradation by REXO2 Maintains Promoter Specificity in Mammalian Mitochondria. Mol Cell 76:784-796.e6 (2019). PubMed: 31588022