Anti-RGS17 / RGSZ2 antibody (ab150814)
Key features and details
- Rabbit polyclonal to RGS17 / RGSZ2
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RGS17 / RGSZ2 antibody
See all RGS17 / RGSZ2 primary antibodies -
Description
Rabbit polyclonal to RGS17 / RGSZ2 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to amino acids 9-106 of Human RGS17/ RGSZ2 (UnIProt ID: Q9UGC6)
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Positive control
- IHC-P: Human testis tissue. WB: Transfected HEK293T whole cell lysates.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine) -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab150814 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 24 kDa.
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 24 kDa. |
IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds selectively to G(z)-alpha and G(alpha)-i2 subunits, accelerates their GTPase activity and regulates their signaling activities. The G(z)-alpha activity is inhibited by the phosphorylation and palmitoylation of the G-protein. Negatively regulates mu-opioid receptor-mediated activation of the G-proteins. -
Tissue specificity
Predominantly expressed in the cerebellum. Also expressed in the cortex and medulla. Weakly expressed in a number of peripheral tissues notably spleen, lung and leukocytes. -
Sequence similarities
Contains 1 RGS domain. -
Post-translational
modificationsFatty acylated. Heavily palmitoylated in the cysteine string motif.
N- and O-glycosylated in synapsomal membranes.
Serine phosphorylated in synapsomal membranes.
Sumoylated by SUMO1 and SUM02 in synaptosomes. The sumoylated forms act as a scaffold for sequestering mu-opioid receptor-activated G(alpha) subunits. -
Cellular localization
Membrane. Nucleus. Cytoplasm. Shuttles between the cytoplasm/cell membrane and the nucleus. Anchored to the membrane through palmitoylation. - Information by UniProt
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Database links
- Entrez Gene: 26575 Human
- Omim: 607191 Human
- SwissProt: Q9UGC6 Human
- Unigene: 166313 Human
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Alternative names
- hRGS17 antibody
- Regulator of G protein signalling 17 antibody
- Regulator of G protein signalling Z2 antibody
see all
Images
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Immunohistochemical analysis of human testis tissue labeling RGS17 / RGSZ2 in the nucleus of a subset of cells in seminiferus ducts with ab150814 at at a 1/50 dilution.
Performed heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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All lanes : Anti-RGS17 / RGSZ2 antibody (ab150814) at 0.4 µg/ml
Lane 1 : Control (vector only transfected) - HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : RGS17 over-expression - HEK-293T whole cell lysate, Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa)
Predicted band size: 24 kDa
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab150814 has not yet been referenced specifically in any publications.