Recombinant Anti-RGS22 antibody [EPR7005] (ab131048)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7005] to RGS22
- Suitable for: WB
- Reacts with: Rat
Related conjugates and formulations
Overview
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Product name
Anti-RGS22 antibody [EPR7005]
See all RGS22 primary antibodies -
Description
Rabbit monoclonal [EPR7005] to RGS22 -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details
Unsuitable for: IHC-P or IP -
Species reactivity
Reacts with: Rat
Predicted to work with: Mouse, Human -
Immunogen
Synthetic peptide within Human RGS22 aa 200-300. The exact sequence is proprietary.
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Positive control
- Rat testis lysate
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General notes
This antibody was developed as part of a collaboration with the lab of Damian Romero at the University of Mississippi Medical Center.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 1.30 x 10 -10 M Learn more about KD -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR7005 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab131048 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 147 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 150 kDa (predicted molecular weight: 147 kDa). |
Target
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Function
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. -
Sequence similarities
Contains 2 RGS domains. - Information by UniProt
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Database links
- Entrez Gene: 26166 Human
- Entrez Gene: 626596 Mouse
- Entrez Gene: 500853 Rat
- SwissProt: Q8NE09 Human
- SwissProt: G3UYX5 Mouse
- Unigene: 120021 Human
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Alternative names
- FLJ40080 antibody
- FLJ75004 antibody
- MGC102908 antibody
see all
Images
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Anti-RGS22 antibody [EPR7005] (ab131048) at 1/1000 dilution + Rat testis lysate at 10 µg
Secondary
HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 147 kDa
Observed band size: 150 kDa why is the actual band size different from the predicted?
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab131048 has been referenced in 1 publication.
- Cao SJ et al. Suppressive effect of YY1-mediated RGS22 regulation on the proliferation, migration and invasion of pancreatic ductal adenocarcinoma. Oncol Lett 24:457 (2022). PubMed: 36380881