Anti-RHCG antibody (ab187904)
Key features and details
- Rabbit polyclonal to RHCG
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-RHCG antibody
See all RHCG primary antibodies -
Description
Rabbit polyclonal to RHCG -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment corresponding to Human RHCG aa 1-100.
Database link: Q9UBD6 -
Positive control
- WB: Human plasma, Human tonsil. IHC-P: Human tonsil, Esophagus, kidney tissues.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab187904 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 53 kDa.
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IHC-P |
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 53 kDa. |
IHC-P
1/1000 - 1/2500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Functions as an electroneutral and bidirectional ammonium transporter. May regulate transepithelial ammonia secretion. -
Tissue specificity
Expressed in brain, testis, placenta, pancreas, esophagus and prostate. Expressed in squamous epithelial tissues (at protein level). According to PubMed:11062476, specifically expressed in kidney. -
Sequence similarities
Belongs to the ammonium transporter (TC 2.A.49) family. Rh subfamily. -
Developmental stage
Specifically expressed in fetal kidney. -
Post-translational
modificationsN-glycosylated. -
Cellular localization
Apical cell membrane. Also detected at the basolateral membrane and in subapical vesicles. - Information by UniProt
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Database links
- Entrez Gene: 51458 Human
- Omim: 605381 Human
- SwissProt: Q9UBD6 Human
- Unigene: 459284 Human
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Alternative names
- Ammonium transporter Rh type C antibody
- C15orf6 antibody
- CDRC2 antibody
see all
Images
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All lanes : Anti-RHCG antibody (ab187904) at 1/100 dilution
Lane 1 : Human cell line RT-4 lysate
Lane 2 : Human cell line U-251MG sp lysate
Lane 3 : Human plasma (IgG/HSA depleted)
Lane 4 : Human liver tissue lysate
Lane 5 : Human tonsil tissue lysate
Developed using the ECL technique.
Predicted band size: 53 kDa
Exposure time: 1 minute -
Immunohistochemical analysis showing RHCG staining in paraffin embedded Human Esophagus tissue using ab187904 at 1/1000 dilution showing positive staining in squamous epithelial cells.
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Immunohistochemical analysis showing RHCG staining in paraffin embedded Human tonsil tissue using ab187904 at 1/1000 dilution showing positive staining in squamous epithelial cells.
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Immunohistochemical analysis showing RHCG staining in paraffin embedded Human kidney tissue using ab187904 at 1/1000 dilution showing positive staining in distal tubule cells.
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Immunohistochemical analysis of paraffin embedded Human lymph node tissue (Negative control) using ab187904 at 1/1000 dilution showing no staining.
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Immunohistochemical analysis of paraffin embedded Human pancrease tissue (Negative control) using ab187904 at 1/1000 dilution showing no staining.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab187904 has been referenced in 1 publication.
- Zhang K et al. CircATIC inhibits esophageal carcinoma progression and promotes radiosensitivity by elevating RHCG through sponging miR-10-3p. Thorac Cancer 13:934-946 (2022). PubMed: 35307984