Anti-RHEB antibody (ab109914)
Key features and details
- Rabbit polyclonal to RHEB
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
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Overview
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Product name
Anti-RHEB antibody
See all RHEB primary antibodies -
Description
Rabbit polyclonal to RHEB -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Rabbit, Horse, Cow, Pig, Chimpanzee, Macaque monkey, Gorilla -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- This antibody gave a positive signal in the following whole cell lysates: Raji; SHSY5Y; EGF treated A431; Jurkat.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109914 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 20 kDa). |
Target
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Function
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity. -
Tissue specificity
Ubiquitous. Highest levels observed in skeletal and cardiac muscle. -
Sequence similarities
Belongs to the small GTPase superfamily. Rheb family. -
Post-translational
modificationsFarnesylation is important for efficiently activating mTORC1-mediated signaling. -
Cellular localization
Cell membrane. - Information by UniProt
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Database links
- Entrez Gene: 528204 Cow
- Entrez Gene: 6009 Human
- Entrez Gene: 26954 Rat
- Omim: 601293 Human
- SwissProt: Q56JV3 Cow
- SwissProt: Q15382 Human
- SwissProt: Q62639 Rat
- Unigene: 283521 Human
see all -
Alternative names
- Ras homolog enriched in brain 2, formerly antibody
- GTP binding protein Rheb antibody
- GTP-binding protein Rheb antibody
see all
Images
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All lanes : Anti-RHEB antibody (ab109914) at 1 µg/ml
Lane 1 : Raji (Human Burkitt's lymphoma cell line) Whole Cell Lysate
Lane 2 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate
Lane 3 : EGF-Stimulated A431 Whole Cell Lysate
Lane 4 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 42 kDa, 49 kDa, 95 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab109914 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab109914 has not yet been referenced specifically in any publications.