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ab39861 |
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Read our guarantee »Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Anti-RHEB antibody
See all RHEB products (6) ...
Rabbit polyclonal to RHEB
ab25873 recognises Rheb (Ras homolog enriched in brain).
IHC-P, WBmore details
Reacts with
Mouse, Rat, Human
Predicted to work with
Fruit fly (Drosophila melanogaster)
A 15 amino acid peptide from the middle region of Human Rheb.
Mouse brain cell lysate
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration information loading...
Immunogen affinity purified
Polyclonal
IgG
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Our Abpromise guarantee covers the use of ab25873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/ml
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 20 kDa.Can be blocked with RHEB peptide (ab39861).
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
Belongs to the small GTPase superfamily. Rheb family.
Farnesylation is important for efficiently activating mTORC1-mediated signaling.
Cell membrane.
Target information above from: UniProt accessionQ15382
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - RHEB antibody (ab25873)

Predicted band size : 20 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Western blot analysis of Rheb in mouse brain cell
lysate with ab25873 at (A) 1, (B) 2, and (C) 4 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RHEB antibody (ab25873)

ab25873 at 2µg/ml staining RHEB in mouse brain tissue by IHC
This product has been referenced in:
See all 2 publications for this product
Publishing research using ab25873? Please let us know so that we can cite the reference in this datasheet
Concentration of lot no. is
Concentration not available for this lot.
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Predicted band size : 20 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Western blot analysis of Rheb in mouse brain cell
lysate with ab25873 at (A) 1, (B) 2, and (C) 4 ug/ml.

ab25873 at 2µg/ml staining RHEB in mouse brain tissue by IHC
1
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