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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-RHEB antibody
See all RHEB products (6) ...
Description
Rabbit polyclonal to RHEB
Specificity
ab25873 recognises Rheb (Ras homolog enriched in brain).
Tested applications
IHC-P, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Predicted to work with
Fruit fly (Drosophila melanogaster)
Immunogen
A 15 amino acid peptide from the middle region of Human Rheb.
Positive control
Mouse brain cell lysate
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab25873 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: Use a concentration of 2 µg/ml
WB: Use a concentration of 1 µg/mlPredicted molecular weight: 20 kDa.Can be blocked with RHEB peptide (ab39861).
Target
Function
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
Tissue specificity
Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
Sequence similarities
Belongs to the small GTPase superfamily. Rheb family.
Post-translational
modifications
Farnesylation is important for efficiently activating mTORC1-mediated signaling.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ15382
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GTP binding protein Rheb antibody
- GTP-binding protein Rheb antibody
- MGC111559 antibody
see all
Database links
- Entrez Gene: 6009 Human
- Entrez Gene: 19744 Mouse
- Entrez Gene: 26954 Rat
- Omim: 601293 Human
- SwissProt: Q9VND8 Fruit fly (Drosophila melanogaster)
- SwissProt: Q15382 Human
- SwissProt: Q921J2 Mouse
- SwissProt: Q62639 Rat
see all
Anti-RHEB antibody images:
Western blot - RHEB antibody (ab25873)
Predicted band size : 20 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
Western blot analysis of Rheb in mouse brain cell
lysate with ab25873 at (A) 1, (B) 2, and (C) 4 ug/ml.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RHEB antibody (ab25873)
ab25873 at 2µg/ml staining RHEB in mouse brain tissue by IHC
References for Anti-RHEB antibody (ab25873)
This product has been referenced in:
- Karbowniczek Met al. The evolutionarily conserved TSC/Rheb pathway activates Notch in tuberous sclerosis complex and Drosophila external sensory organ development. J Clin Invest 120:93-102 (2010).Read more (PubMed: 20038815) »
- Acosta-Jaquez HAet al. Site-specific mTOR phosphorylation promotes mTORC1-mediated signaling and cell growth. Mol Cell Biol 29:4308-24 (2009). WB.Read more (PubMed: 19487463) »
See all 2 publications for this product
Publishing research using ab25873? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"