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Western blot - RHEB antibody (ab25873)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RHEB antibody (ab25873)

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Product Name
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RHEB antibody
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See all RHEB antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RHEB
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Immunogen
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A 15 amino acid peptide from the middle region of Human Rheb.
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab25873 recognises Rheb (Ras homolog enriched in brain).
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Tested applications
(see key)
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IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions IHC-P: Use at a concentration of 2 µg/ml. WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. Can be blocked with RHEB peptide (ab39861).
Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Mouse brain cell lysate
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Cellular localization
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Cell Membrane; lipid anchor, cytoplasmic side
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Relevance
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Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.
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Database links
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The links below go to external sites and will open in a new browser window
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide Constituents: PBS Material safety datasheet (MSDS) for this product: Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RHEB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RHEB antibody images, references, products related to ab25873 and other tools.
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RHEB antibody images:
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - RHEB antibody (ab25873)
ab25873 at 2µg/ml staining RHEB in mouse brain tissue by IHC
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References for RHEB antibody (ab25873)
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This product has been used in: (two most recent references)
Karbowniczek M et al. The evolutionarily conserved TSC/Rheb pathway activates Notch in tuberous sclerosis complex and Drosophila external sensory organ development. J Clin Invest 120:93-102 (2010). PubMed: 20038815
Acosta-Jaquez HA et al. Site-specific mTOR phosphorylation promotes mTORC1-mediated signaling and cell growth. Mol Cell Biol 29:4308-24 (2009). WB. PubMed: 19487463
If you publish research using ab25873 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to RHEB
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RHEB antibody - more information
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Customer reviews (feedback) regarding RHEB antibody |
Customer FAQs regarding RHEB antibody |
Protocols for RHEB antibody |
Price and availability of products related to RHEB antibody |
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RHEB antibody - related products:
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RHEB antibody - other tools:
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Contact Abcam with a Technical Enquiry about RHEB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"