RHEB antibody (ab25873)

Product Name 

RHEB antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to RHEB

Immunogen 

A 15 amino acid peptide from the middle region of Human Rheb.

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

ab25873 recognises Rheb (Ras homolog enriched in brain).

Tested applications 
(see key)

IHC-P, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
IHC-P: Use at a concentration of 2 µg/ml.
WB: Use at a concentration of 1 µg/ml. Predicted molecular weight: 20 kDa. Can be blocked with RHEB peptide (ab39861).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Mouse brain cell lysate

Cellular localization 

Cell Membrane; lipid anchor, cytoplasmic side

Research areas 

Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family

Relevance 

Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: PBS

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

1.000 mg/ml

Storage instructions 

Store at +4°C.

See below for RHEB antibody images, references, products related to ab25873 and other tools.

RHEB antibody images:

References for RHEB antibody (ab25873)

  This product has been used in: (two most recent references)

Karbowniczek M et al. The evolutionarily conserved TSC/Rheb pathway activates Notch in tuberous sclerosis complex and Drosophila external sensory organ development. J Clin Invest 120:93-102 (2010). PubMed: 20038815

Acosta-Jaquez HA et al. Site-specific mTOR phosphorylation promotes mTORC1-mediated signaling and cell growth. Mol Cell Biol 29:4308-24 (2009). WB. PubMed: 19487463
If you publish research using ab25873 please let us know so that we can cite the reference on this datasheet.

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