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Western blot - RHEB antibody (ab25976)
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Product Name
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RHEB antibody
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See all RHEB antibodies (3)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RHEB
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Immunogen
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Synthetic peptide, corresponding to 14 amino acids at the N terminus of Human RHEB.
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Reacts with
(species key)
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Hu, Ms, Rat
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Tested applications
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 2 - 4 µg/ml. Detects a band of approximately 20 kDa (predicted molecular weight: 20 kDa). Can be blocked with RHEB peptide (ab39862).
Post-translationally modified Rheb is sometimes observed at higher molecular weight.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Rat heart cell lysate can be used as positive control.
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Cellular localization
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Cell Membrane; lipid anchor, cytoplasmic side
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Research areas
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Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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Relevance
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Rheb (Ras homolog enriched in brain) is an evolutionarily conserved member of the Ras family of small GTP binding proteins originally found to be rapidly induced by synaptic activity in the hippocampus following seizure. While it is expressed at relatively high levels in the brain, Rheb is widely expressed in other tissues and may be induced by growth factor stimulation. Similar to other family members, Rheb triggers activation of the Raf-MEK-MAPK pathway. Biochemical and genetic studies demon-strate that Rheb has an important role in regulating the insulin/Target of rapamycin (TOR) signaling pathway. TOR is a serine/threonine protein kinase that acts as a sensor for ATP and amino acids, balancing the availability of nutrients with protein translation and cell growth. A dimeric protein complex termed TSC1/TSC2 indirectly inhibits TOR activity by inhibiting Rheb via the GAP activity of TSC2.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RHEB antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RHEB antibody images, references, products related to ab25976 and other tools.
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RHEB antibody images:
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 Western blot - RHEB antibody (ab25976)
Lane 1 : RHEB antibody (ab25976) at 2 µg/ml
Lane 2 : RHEB antibody (ab25976) at 4 µg/ml
Lane 1 : Rat heart cell lysate
Lane 2 : Rat heart cell lysate
Predicted band size : 20 kDa
Observed band size : 20 kDa
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Search PubMed (MEDLINE) for references to RHEB
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RHEB antibody - more information
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Customer reviews (feedback) regarding RHEB antibody |
Customer FAQs regarding RHEB antibody |
Protocols for RHEB antibody |
Price and availability of products related to RHEB antibody |
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RHEB antibody - related products:
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RHEB antibody - other tools:
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Contact Abcam with a Technical Enquiry about RHEB antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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