Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
Anti-RHEB antibody
See all RHEB products (6) ...
Description
Mouse monoclonal to RHEB
Tested applications
ELISA, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Recombinant full length Human RHEB protein (AAH16155, 1 a.a. ~ 185 a.a) with tag.
Positive control
Jurkat cell lysate; Recombinant tagged full length human RHEB.
Properties
Form
Liquid
Storage instructions
Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
Storage buffer
Preservative: None
Constituents: 1X PBS, pH 7.2
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Monoclonal
Isotype
IgG1
Light chain type
kappa
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Applications
Show applications key
Our Abpromise guarantee covers the use of ab76629 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ELISA: Use at an assay dependent dilution. (Detection limit for recombinant tagged RHEB is approximately 3ng/ml if used as a capture antibody. )
WB: Use a concentration of 1 - 5 µg/ml.Predicted molecular weight: 20 kDa.
Target
Function
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
Tissue specificity
Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
Sequence similarities
Belongs to the small GTPase superfamily. Rheb family.
Post-translational
modifications
Farnesylation is important for efficiently activating mTORC1-mediated signaling.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ15382
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- GTP binding protein Rheb antibody
- GTP-binding protein Rheb antibody
- MGC111559 antibody
see all
Database links
- Entrez Gene: 6009 Human
- Entrez Gene: 19744 Mouse
- Entrez Gene: 26954 Rat
- Omim: 601293 Human
- SwissProt: Q15382 Human
- SwissProt: Q921J2 Mouse
- SwissProt: Q62639 Rat
- Unigene: 283521 Human
see all
Anti-RHEB antibody images:
Western blot - RHEB antibody (ab76629)
Anti-RHEB antibody (ab76629) at 1 µg/ml + Recombinant tagged full length human RHEB at 0.2 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/5000 dilution
Predicted band size : 20 kDa
Observed band size : 46 kDa (why is the actual band size different from the predicted?)
Western blot against tagged recombinant protein immunogen. Predicted band size of immunogen is 46kDa.
Western blot - RHEB antibody (ab76629)
Anti-RHEB antibody (ab76629) at 1 µg/ml + Jurkat cell lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size : 20 kDa
Observed band size : 17 kDa (why is the actual band size different from the predicted?)
References for Anti-RHEB antibody (ab76629)
ab76629 has not yet been referenced specifically in any publications.
Publishing research using ab76629? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"