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Products:Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
Overview
Product name
RHEB protein (His tag)
See all RHEB products (5) ...
Protein description
RHEB full length protein (Human) with N-terminal His tag, MW ~21kDa. Expressed using baculovirus in Sf9 cells.
Properties
Purification notes
Purity : >95% by densitometry.
Form
Liquid
Storage instructions
Shipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 150mM Imidazole
Constituents: 25% Glycerol, 50mM Sodium phosphate, 300mM Sodium chloride, 0.2mM DTT, pH 7.0
Concentration
Concentration information loading...
Research areas
Cancer >> Signal transduction >> G protein signaling >> Small G proteins >> Ras family
Signal Transduction >> Signaling Pathway >> G Protein Signaling >> Small G Proteins >> Ras Family
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SDS-PAGE - RHEB protein (His tag) (ab56271)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab56271 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
SDS-PAGE: Use at an assay dependent dilution.
Protein info
Function
Stimulates the phosphorylation of S6K1 and EIF4EBP1 through activation of mTORC1 signaling. Activates the protein kinase activity of mTORC1. Has low intrinsic GTPase activity.
Tissue specificity
Ubiquitous. Highest levels observed in skeletal and cardiac muscle.
Sequence similarities
Belongs to the small GTPase superfamily. Rheb family.
Post-translational
modifications
Farnesylation is important for efficiently activating mTORC1-mediated signaling.
Cellular localization
Cell membrane.
Target information above from: UniProt accessionQ15382
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
RHEB protein (His tag) images:
References for RHEB protein (His tag) (ab56271)
ab56271 has not yet been referenced specifically in any publications.
Publishing research using ab56271? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"