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Overview

  • Product nameRHOG protein (Tagged-His Tag)See all RHOG proteins and peptides ...
  • Protein descriptionRecombinant fragment, corresponding to amino acids 32-191 of Human RHOG with an N terminal His tag; MWt 19kDa (UniProt P84095).
  • Expression hostE. coli

    • Properties

  • Purification notesPurity level >85%
    This protein was expressed as an N-terminal His-tag fusion protein using Escherichia coli, and purified using Immobilized Metal Ion Affinity Chromatography. In some cases, smaller protein fragments may be present in addition to the intended expression product as a result of premature termination during translation in E. coli and subsequent co-purification via the His-tag. In some cases purified proteins run at a molecular weight different to the theoretically calculated molecular weight. This may be as a result of unequally distributed charges in the amino acid sequence. Alternatively, dimerisation of the expression product can occur under oxygen limitation during expression/cultivation.
  • FormLyophilised:Reconstitute with 104 µl aqua dest.
  • Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.
  • Storage bufferPreservative: None
    Constituents: 0.5% Trehalose, 6M Urea, 100mM Sodium phosphate, 10mM Sodium chloride, pH 4.5
  • Concentration information loading...
  • Sequence notesYIPTVFDNYSAQSAVDGRTVNLNLWDTAGQEEYDRLRTLS YPQTNVFVIC FSIASPPSYENVRHKWHPEVCHHCPDVP ILLVGTKKDLRAQPDTLRRLKE QGQAPITPQQGQALAK QIHAVRYLECSALQQDGVKEVFAEAVRAVLNPTP IKRG RSCILL
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab91666 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
  • Application notesSDS-PAGE: Use at an assay dependent dilution.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        ARHGRas homolog family member GRho G
        Rho related GTP binding protein RhoG precursorRho-related GTP-binding protein RhoGRHOGRHOG_HUMAN
      see all
  • FunctionRequired for the formation of membrane ruffles during macropinocytosis. Required for the formation of cup-like structures during trans-endothelial migration of leukocytes. In case of Salmonella enterica infection, activated by SopB and ARHGEF26/SGEF, which induces cytoskeleton rearrangements and promotes bacterial entry.
  • Sequence similaritiesBelongs to the small GTPase superfamily. Rho family.
  • Cellular localizationCell membrane.

    • Target information above from: UniProt accession P84095 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    RHOG protein (Tagged-His Tag) images

    • SDS-PAGE - RHOG protein (Tagged-His Tag) (ab91666)
      SDS-PAGE - RHOG protein (Tagged-His Tag) (ab91666)
      The image shows an electrophoretic assay performed using an Agilent 5100 ALP. In some images coloured control bands can be seen at 15 kDa (green) and/or 240 kDa (purple). The protein-specific band is blue.

    References for RHOG protein (Tagged-His Tag) (ab91666)

    ab91666 has not yet been referenced specifically in any publications.

    Publishing research using ab91666 ? Please let us know so that we can cite the reference in this datasheet

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