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Western blot - RICTOR antibody (ab32892)
(enlarge)
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Product Name
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RICTOR antibody
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See all RICTOR antibodies (7)...
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Product type
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Primary antibodies
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Description
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Goat polyclonal to RICTOR
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Immunogen
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Synthetic peptide: (C)KQPIVDTSAES, corresponding to C terminal amino acids 1698-1708 of Human RICTOR
BLAST '(C)KQPIVDTSAES' with  or 
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Predicted to react
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Hu, Ms, Dog
(Due to sequence homology)
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Tested applications
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IHC-P
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Abreviews
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Application notes
(see key)
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Recommended dilutions
Peptide ELISA: antibody detection limit dilution 1:4,000.
IHC-P: Use at a concentration of 2 - 3 µg/ml.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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RICTOR is a component of a protein complex [mTORC2] that integrates nutrient- and growth factor-derived signals to regulate cell growth. Rictor is a key component of mTORC2 which plays an essential role in Akt phosphorylation and signaling.
Deregulation of Akt/protein kinase B (PKB) is implicated in the pathogenesis of cancer and diabetes. Rictor-mTOR may serve as a drug target in tumors that have lost the expression of PTEN, a tumor suppressor that opposes Akt/PKB activation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Goat
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
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Form
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Liquid
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Concentration
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0.500 mg/ml
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Storage instructions
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Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RICTOR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RICTOR antibody images, references, products related to ab32892 and other tools.
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RICTOR antibody images:
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 Western blot - RICTOR antibody (ab32892)
Ab32892 (2.5ug/ml) staining human RICTOR in human kidney tissue by immunohistochemistry using paraffin embedded tissue.
Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
In paraffin embedded Kidney shows strong cytoplasmic
staining of epithelial cells in distal convoluted tubules.
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Search PubMed (MEDLINE) for references to RICTOR
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RICTOR antibody - more information
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Customer reviews (feedback) regarding RICTOR antibody |
Customer FAQs regarding RICTOR antibody |
Protocols for RICTOR antibody |
Price and availability of products related to RICTOR antibody |
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RICTOR antibody - related products:
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RICTOR antibody - other tools:
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Contact Abcam with a Technical Enquiry about RICTOR antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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