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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-RICTOR antibody
See all RICTOR products (11) ...
Description
Goat polyclonal to RICTOR
Tested applications
ICC/IF, ELISA, IHC-Pmore details
Cross reactivity
Predicted to work with
Mouse, Dog, Human
Immunogen
Synthetic peptide: (C)KQPIVDTSAES, corresponding to C terminal amino acids 1698-1708 of Human RICTOR
(C)KQPIVDT SAES
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline. pH 7.3
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Purification notes
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Western blot - RICTOR antibody (ab32892)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32892 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: 1/100
ELISA: 1/4000(Peptide ELISA detection limit dilution 1:4,000.)
IHC-P: Use a concentration of 2 - 3 µg/ml.
Target
Function
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Sequence similarities
Belongs to the pianissimo family.
Post-translational
modifications
Phosphorylated by MTOR; when part of mTORC2.
Target information above from: UniProt accessionQ6R327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AVO3 antibody
- AVO3 homolog antibody
- DKFZp686B11164 antibody
see all
Database links
- Entrez Gene: 253260 Human
- Entrez Gene: 78757 Mouse
- Omim: 609022 Human
- SwissProt: Q6R327 Human
- SwissProt: Q6QI06 Mouse
- Unigene: 407926 Human
- Unigene: 275811 Mouse
Anti-RICTOR antibody images:
Western blot - RICTOR antibody (ab32892)
Ab32892 (2.5ug/ml) staining human RICTOR in human kidney tissue by immunohistochemistry using paraffin embedded tissue.
Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
In paraffin embedded Kidney shows strong cytoplasmic
staining of epithelial cells in distal convoluted tubules.
References for Anti-RICTOR antibody (ab32892)
This product has been referenced in:
- Joha Set al. GILZ inhibits the mTORC2/AKT pathway in BCR-ABL(+) cells. Oncogene : (2011).Read more (PubMed: 21804606) »
See 1 publication for this product
Publishing research using ab32892? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"