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Western blot - RICTOR antibody (ab70373)
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Product Name
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RICTOR antibody
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See all RICTOR antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RICTOR
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Immunogen
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Synthetic peptide corresponding to a region between residues 1 and 50 of human RICTOR.
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Reacts with
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Hu
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Does not react with
(species key)
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Ms
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Tested applications
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 3-5µg/mg of lysate.
WB: 1/2000 - 1/10000. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysate from HeLa cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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RICTOR is a component of a protein complex [mTORC2] that integrates nutrient- and growth factor-derived signals to regulate cell growth. Rictor is a key component of mTORC2 which plays an essential role in Akt phosphorylation and signaling.
Deregulation of Akt/protein kinase B (PKB) is implicated in the pathogenesis of cancer and diabetes. Rictor-mTOR may serve as a drug target in tumors that have lost the expression of PTEN, a tumor suppressor that opposes Akt/PKB activation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS, 8mM Phosphate, 60mM Citrate, 150mM Tris, pH 7-8 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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1.000 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RICTOR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RICTOR antibody images, references, products related to ab70373 and other tools.
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RICTOR antibody images:
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 Western blot - RICTOR antibody (ab70373)
All lanes : RICTOR antibody (ab70373) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 192 kDa
Observed band size : 192 kDa
Additional bands at : 160 kDa,300 kDa,80 kDa. We are unsure as to the identity of these extra bands.
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References for RICTOR antibody (ab70373)
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This product has been used in: (two most recent references)
Yu Y & Alwine JC Interaction between simian virus 40 large T antigen and insulin receptor substrate 1 is disrupted by the K1 mutation, resulting in the loss of large T antigen-mediated phosphorylation of Akt. J Virol 82:4521-6 (2008). PubMed: 18305032
Nami M et al. Nuclear forkhead box O1 controls and integrates key signaling pathways in hepatocytes. Endocrinology 148:2424-34 (2007). PubMed: 17303659
If you publish research using ab70373 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to RICTOR
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RICTOR antibody - more information
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Customer reviews (feedback) regarding RICTOR antibody |
Customer FAQs regarding RICTOR antibody |
Protocols for RICTOR antibody |
Price and availability of products related to RICTOR antibody |
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RICTOR antibody - related products:
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RICTOR antibody - other tools:
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Contact Abcam with a Technical Enquiry about RICTOR antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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