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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-RICTOR antibody
See all RICTOR products (11) ...
Description
Rabbit polyclonal to RICTOR
Specificity
We have data to indicate that this antibody may not cross react with Mouse. However, this has not been conclusively tested and expression levels may vary in certain cell lines/tissues.
Tested applications
WB, IPmore details
Cross reactivity
Reacts with
Human
Immunogen
Synthetic peptide corresponding to a region between residues 1 and 50 of human RICTOR.
Positive control
Whole cell lysate from HeLa cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Western blot - RICTOR antibody (ab70373)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70373 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
IP: Use at 3-5 µg/mg of lysate.
Target
Function
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Sequence similarities
Belongs to the pianissimo family.
Post-translational
modifications
Phosphorylated by MTOR; when part of mTORC2.
Target information above from: UniProt accessionQ6R327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AVO3 antibody
- AVO3 homolog antibody
- DKFZp686B11164 antibody
see all
Database links
- Entrez Gene: 253260 Human
- Omim: 609022 Human
- SwissProt: Q6R327 Human
- Unigene: 407926 Human
Anti-RICTOR antibody images:
Western blot - RICTOR antibody (ab70373)
All lanes : Anti-RICTOR antibody (ab70373) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size : 192 kDa
Observed band size : 192 kDa
Additional bands at : 160 kDa,300 kDa,80 kDa. We are unsure as to the identity of these extra bands.
References for Anti-RICTOR antibody (ab70373)
This product has been referenced in:
- Yu Y & Alwine JC Interaction between simian virus 40 large T antigen and insulin receptor substrate 1 is disrupted by the K1 mutation, resulting in the loss of large T antigen-mediated phosphorylation of Akt. J Virol 82:4521-6 (2008).Read more (PubMed: 18305032) »
See 1 publication for this product
Publishing research using ab70373? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"