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If your product does not perform as described on this datasheet, we will refund or replace your product...
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Western blot - RICTOR antibody (ab70374)
All lanes : Anti-RICTOR antibody (ab70374) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 2.5 µg
Lane 3 : HeLa whole cell lysate at 1 µg
Lane 4 : HeLa whole cell lysate at 0.5 µg
Lane 5 : NIH3T3 cells at 50 µg
Predicted band size : 192 kDa
Observed band size : 192 kDa
Additional bands at : 78 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - RICTOR antibody (ab70374)
Detection of Human RICTOR by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70374 at 3 µg/mg for IP (Lane 1) and at 0.04 µg/ml for subsequent WB detection. Lane 2 represents control rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-RICTOR antibody(ab70374)
ab70374 (2µg/ml) staining RICTOR in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of glandular epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
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