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Western blot - RICTOR antibody (ab70374)
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Immunoprecipitation - RICTOR antibody (ab70374)
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Product Name
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RICTOR antibody
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See all RICTOR antibodies (7)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RICTOR
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Immunogen
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Synthetic peptide corresponding to a region between residue 1650 and the C-terminus (residue 1708) of human RICTOR.
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Reacts with
(species key)
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Hu, Ms
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Specificity
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Initial tests for reactivity with mouse RICTOR from NIH3T3 cells suggest that higher amounts of lysate and less dilution of ab70374 are required for brief exposure times.
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Tested applications
(see key)
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IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
IP: Use at 3-5µg/mg of lysate.
WB: 1/5000 - 1/15000. Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Whole cell lysates from HeLa and NIH3T3 cells.
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Cellular localization
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Cytoplasmic
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Research areas
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Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
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Relevance
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RICTOR is a component of a protein complex [mTORC2] that integrates nutrient- and growth factor-derived signals to regulate cell growth. Rictor is a key component of mTORC2 which plays an essential role in Akt phosphorylation and signaling.
Deregulation of Akt/protein kinase B (PKB) is implicated in the pathogenesis of cancer and diabetes. Rictor-mTOR may serve as a drug target in tumors that have lost the expression of PTEN, a tumor suppressor that opposes Akt/PKB activation.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.200 mg/ml
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Storage instructions
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Store at +4°C.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RICTOR antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RICTOR antibody images, references, products related to ab70374 and other tools.
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RICTOR antibody images:
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 Western blot - RICTOR antibody (ab70374)
All lanes : RICTOR antibody (ab70374) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 2.5 µg
Lane 3 : HeLa whole cell lysate at 1 µg
Lane 4 : HeLa whole cell lysate at 0.5 µg
Lane 5 : NIH3T3 cells at 50 µg
Predicted band size : 192 kDa
Observed band size : 192 kDa
Additional bands at : 78 kDa,80 kDa. We are unsure as to the identity of these extra bands.
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Immunoprecipitation - RICTOR antibody (ab70374)
Detection of Human RICTOR by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70374 at 3 µg/mg for IP (Lane 1) and at 0.04 µg/ml for subsequent WB detection. Lane 2 represents control rabbit IgG.
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Search PubMed (MEDLINE) for references to RICTOR
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RICTOR antibody - more information
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Customer reviews (feedback) regarding RICTOR antibody |
Customer FAQs regarding RICTOR antibody |
Protocols for RICTOR antibody |
Price and availability of products related to RICTOR antibody |
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RICTOR antibody - related products:
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RICTOR antibody - other tools:
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Contact Abcam with a Technical Enquiry about RICTOR antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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