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Products:Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Overview
Product name
Anti-RICTOR antibody
See all RICTOR products (11) ...
Description
Rabbit polyclonal to RICTOR
Specificity
Initial tests for reactivity with mouse RICTOR from NIH3T3 cells suggest that higher amounts of lysate and less dilution of ab70374 are required for brief exposure times.
Tested applications
WB, IP, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide corresponding to a region between residue 1650 and the C-terminus (residue 1708) of human RICTOR.
Positive control
Whole cell lysates from HeLa and NIH3T3 cells.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Signal Transduction >> Adapters >> Cytoplasmic
Signal Transduction >> Protein Phosphorylation >> pSer / pThr
Cell Biology >> Cell Cycle >> Cell Cycle Inhibitors >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70374 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/5000 - 1/15000.Detects a band of approximately 192 kDa (predicted molecular weight: 192 kDa).
IP: Use at 3-5 µg/mg of lysate.
IHC-P: Use a concentration of 2 µg/ml(Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.)
Target
Function
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development.
Sequence similarities
Belongs to the pianissimo family.
Post-translational
modifications
Phosphorylated by MTOR; when part of mTORC2.
Target information above from: UniProt accessionQ6R327
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- AVO3 antibody
- AVO3 homolog antibody
- DKFZp686B11164 antibody
see all
Database links
- Entrez Gene: 253260 Human
- Entrez Gene: 78757 Mouse
- Omim: 609022 Human
- SwissProt: Q6R327 Human
- SwissProt: Q6QI06 Mouse
- Unigene: 407926 Human
- Unigene: 275811 Mouse
Anti-RICTOR antibody images:
Western blot - RICTOR antibody (ab70374)
All lanes : Anti-RICTOR antibody (ab70374) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 5 µg
Lane 2 : HeLa whole cell lysate at 2.5 µg
Lane 3 : HeLa whole cell lysate at 1 µg
Lane 4 : HeLa whole cell lysate at 0.5 µg
Lane 5 : NIH3T3 cells at 50 µg
Predicted band size : 192 kDa
Observed band size : 192 kDa
Additional bands at : 78 kDa,80 kDa. We are unsure as to the identity of these extra bands.
Immunoprecipitation - RICTOR antibody (ab70374)
Detection of Human RICTOR by Immunoprecipitation in Whole cell lysate from HeLa cells (1 mg for IP, 1/4 of IP loaded) using ab70374 at 3 µg/mg for IP (Lane 1) and at 0.04 µg/ml for subsequent WB detection. Lane 2 represents control rabbit IgG.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-RICTOR antibody(ab70374)
ab70374 (2µg/ml) staining RICTOR in human prostate using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic staining of glandular epithelium.
Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
References for Anti-RICTOR antibody (ab70374)
This product has been referenced in:
- Takai Het al. Tel2 structure and function in the Hsp90-dependent maturation of mTOR and ATR complexes. Genes Dev 24:2019-30 (2010). IP; Mouse.Read more (PubMed: 20801936) »
See 1 publication for this product
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"