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Western blot - RING1 antibody (ab32644)
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Western blot - RING1 antibody (ab32644)
(enlarge)
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Product Name
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RING1 antibody
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See all RING1 antibodies (4)...
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to RING1
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Immunogen
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Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human RING1.
(Peptide available as ab32805.)
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Reacts with
(species key)
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Hu, Ms, Rat
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Specificity
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ab32644 detects isoform 2 of RING1 which is predicted to be ~39 kDa. For detection of isoform 1 of RING1 we recommend the use of ab32807.
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Tested applications
(see key)
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WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 39 kDa (predicted molecular weight: 42,39 kDa).
ab32644 detects isoform 2 of RING1 which is predicted to be ~39 kDa. For detection of isoform 1 of RING1 we recommend the use of ab32807. Please see the figure below for further information.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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This antibody gave a positive control in the following human lysates:
Kidney tissue
Liver tissue
This antibody gave a positive control in the following mouse lysate:
Kidney tissue
This antibody gave a positive control in the following rat lysate:
Kidney Whole Cell Lysate - normal tissue
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Cellular localization
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Nucleus speckle.
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Research areas
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Chromatin and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC1
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Relevance
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This gene belongs to the RING finger family, members of which encode proteins characterized by a RING domain, a zinc binding motif related to the zinc finger domain. The gene product can bind DNA and can act as a transcriptional repressor. It is associated with the multimeric polycomb group protein complex. The gene product interacts with the polycomb group proteins BMI1, EDR1, and CBX4, and colocalizes with these proteins in large nuclear domains. It interacts with the CBX4 protein via its glycine rich C terminal domain. The gene maps to the HLA class II region, where it is contiguous with the RING finger genes FABGL and HKE4.
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Immunogen affinity purified
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Storage buffer
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Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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Batch dependent within range: 0.46 - 1.00 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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At Abcam, we have one centralized database to hold all of our product information, so that everything we know about this RING1 antibody is on this datasheet. But please do contact us if you would like any reassurance! |
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See below for RING1 antibody images, references, products related to ab32644 and other tools.
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RING1 antibody images:
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 Western blot - RING1 antibody (ab32644)
All lanes : RING1 antibody (ab32644) at 1 µg/ml
Lane 1 : Human Kidney Whole Tissue Lysate
Lane 2 : Human Liver Whole Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 42,39 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
ab32644 detects isoform 2 of RING1 which is predicted to be ~39 kDa. Theoretically this antibody should also detect isoform 1 of RING1 (which we see in brain lysate using ab32807) as the epitope for ab32644 is predicted to be present in isoform 1. This is not the case and we suspect that this may be due to either an undocumented modification or variable splicing of the C-terminus of isoform 1 that removes the epitope that ab32644 recognises.
We recommend the use of ab32807 for detection of Isoform 1 of RING1.
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Search PubMed (MEDLINE) for references to RING1
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RING1 antibody - more information
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Customer reviews (feedback) regarding RING1 antibody |
Customer FAQs regarding RING1 antibody |
Protocols for RING1 antibody |
Price and availability of products related to RING1 antibody |
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RING1 antibody - related products:
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RING1 antibody - other tools:
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Contact Abcam with a Technical Enquiry about RING1 antibody |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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