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Products:Epigenetics and Nuclear Signaling >> Chromatin Remodeling >> Polycomb Silencing >> PRC1
Overview
Product name
Anti-RING1 antibody - ChIP Grade
See all RING1 products (7) ...
Description
Rabbit polyclonal to RING1 - ChIP Grade
Specificity
ab32644 detects isoform 2 of RING1 which is predicted to be ~39 kDa. For detection of isoform 1 of RING1 we recommend the use of ab32807.
Tested applications
ChIP, WBmore details
Cross reactivity
Reacts with
Mouse, Rat, Human
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human RING1.
(Peptide available as ab32805.)
Positive control
This antibody gave a positive control in the following human lysates: Kidney tissue Liver tissue This antibody gave a positive control in the following mouse lysate: Kidney tissue This antibody gave a positive control in the following rat lysate: Kidney Whole Cell Lysate - normal tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Applications
Show applications key
Our Abpromise guarantee covers the use of ab32644 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ChIP: Use at an assay dependent dilution. (PubMed: 20237320)
WB: Use a concentration of 1 µg/mlDetects a band of approximately 39 kDa (predicted molecular weight: 42,39 kDa).
Target
Function
Constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.
Pathway
Protein modification; protein ubiquitination.
Sequence similarities
Contains 1 RING-type zinc finger.
Cellular localization
Nucleus speckle.
Target information above from: UniProt accessionQ06587
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Ring1A antibody
- RING finger protein 1 antibody
- Transcription repressor Ring1A antibody
see all
Database links
- Entrez Gene: 6015 Human
- Entrez Gene: 19763 Mouse
- Entrez Gene: 309626 Rat
- Omim: 602045 Human
- SwissProt: Q06587 Human
- SwissProt: O35730 Mouse
- SwissProt: Q6MGB6 Rat
- Unigene: 727565 Human
see all
Anti-RING1 antibody - ChIP Grade images:
Western blot - RING1 antibody (ab32644)
All lanes : Anti-RING1 antibody - ChIP Grade (ab32644) at 1 µg/ml
Lane 1 : Human Kidney Whole Tissue Lysate
Lane 2 : Human Liver Whole Tissue Lysate
Lysates/proteins at 20 µg per lane.
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 42,39 kDa
Observed band size : 39 kDa (why is the actual band size different from the predicted?)
ab32644 detects isoform 2 of RING1 which is predicted to be ~39 kDa. Theoretically this antibody should also detect isoform 1 of RING1 (which we see in brain lysate using ab32807) as the epitope for ab32644 is predicted to be present in isoform 1. This is not the case and we suspect that this may be due to either an undocumented modification or variable splicing of the C-terminus of isoform 1 that removes the epitope that ab32644 recognises.
We recommend the use of ab32807 for detection of Isoform 1 of RING1.
Western blot - RING1 antibody (ab32644)
All lanes : Anti-RING1 antibody - ChIP Grade (ab32644) at 1 µg/ml
Lane 1 : Kidney (Mouse) Tissue Lysate
Lane 2 : Kidney (Rat) Whole Cell Lysate - normal tissue (ab29480)
Lysates/proteins at 10 µg per lane.
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 42,39 kDa
Observed band size : 37 kDa (why is the actual band size different from the predicted?)
References for Anti-RING1 antibody - ChIP Grade (ab32644)
This product has been referenced in:
- Argiropoulos Bet al. Linkage of the potent leukemogenic activity of Meis1 to cell-cycle entry and transcriptional regulation of cyclin D3. Blood 115:4071-82 (2010). ChIP; Mouse.Read more (PubMed: 20237320) »
See 1 publication for this product
Publishing research using ab32644? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"