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ab32805 |
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ab32806 |
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Anti-RING1 antibody (ab32807) at 1 µg/ml + Human Brain Whole Tissue Lysate at 20 µg
Secondary
IR Dye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/15000 dilution
Performed under reducing conditions.
Predicted band size : 42,39 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
The epitope for
An alternative antibody is available (
Anti-RING1 antibody (ab32807) at 1 µg/ml + Brain (Mouse) Tissue Lysate at 10 µg
Secondary
IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size : 42,39 kDa
Observed band size : 42 kDa (why is the actual band size different from the predicted?)
Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
ab32807 specifically detects isoform 1 of RING1 which is predicted to be ~42 kDa.
The epitope for ab32807 is not present in isoform 2 (the ~39 kDa form).
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